We narrowed to 7,638 results for: val

Showing: 5201 - 5220 of 7638 results

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pEGFP-MYO10-HMM

Plasmid

#87256

Purpose

Expression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Expression

Mammalian

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHDM-SARS-CoV-2

Plasmid

#164433

Purpose

Encodes SARS-CoV-2 Spike Protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

Feb. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-CA-c-Src-FLAG

Plasmid

#44654

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed Tyr 530 to Phe

Promoter

CMV promoter

Available Since

May 6, 2013

Availability

Academic Institutions and Nonprofits only

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pSYC-176

Plasmid

#178961

Purpose

Expresses human GFAP fused to 3xFLAG tag in mammalian cells

Depositor

Seok-Yong Choi

Article

Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

Insert

Glial fibrillary acidic protein (GFAP Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV IE94 Promoter

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRecLV105

Plasmid

#107238

Purpose

A lentiviral vector for expression in mammalian cells

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLX317 CALM2

Plasmid

#193684

Purpose

Constitutive lentiviral expression of CALM2

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

CALM2 (CALM2 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-trkB.DN-mCherry

Plasmid

#121502

Purpose

Expresses trkB.T1 fused with mCherry in a cre dependent manner.

Depositor

Marie Carlén

Article

Guyon et al J Neurosci. 2021 Feb 15. pii: JNEUROSCI.

Insert

trkB.T1 (Ntrk2 Rat)

Use

AAV and Cre/Lox

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shYAP1-1

Plasmid

#193692

Purpose

Constitutive lentiviral expression of YAP1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miRFP-6xFLAG

Plasmid

#161614

Purpose

Cell morphology marker construct. Contains a near infrared fluorescent protein miRFP and six FLAG tags.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

miRFP-6xFLAG

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human synapsin 1 promoter

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase INF enhancer vector_ORI_IFIT2

Plasmid

#99321

Purpose

Luciferase validation vector with IFIT2 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

enhancer; chr10: 91060605-91062447

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS

Plasmid

#162610

Purpose

Allows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Expression

Mammalian

Mutation

Contains aa1-155 of mVenus

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTMB341_ZFcharm_Prnp_SCR

Plasmid

#220844

Purpose

AAV genome expressing ZFcharm targeting Prnp; scrambled self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pD40-His/V5-c-MycS62A

Plasmid

#45599

Depositor

Rosalie Sears

Article

Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

Insert

c-Myc S62A (Myc Mouse)

Tags

6xHis and V5

Expression

Mammalian

Mutation

S62A

Promoter

CMV

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
SYKA-c020

Plasmid

#175489

Purpose

Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

Depositor

Opher Gileadi

Article

Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

Insert

SYK (SYK Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-HOXA5

Plasmid

#97040

Purpose

Expresses HOXA5 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Homeobox A5 (HOXA5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86021

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain

Promoter

CMV IE

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG DEPTOR (13×S/T->A)

Plasmid

#21702

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

DEPTOR 13xA (DEPTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

Nonphosphorylatable mutant. 13xA. Also has N204S …

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-KD-c-Src-FLAG

Plasmid

#44653

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed lysine 298 to methionine

Promoter

CMV promoter

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV/myc/mito-mutant-hogg1

Plasmid

#18708

Depositor

David Sidransky

Article

Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

Insert

8-oxoguanine DNA glycosylase (OGG1 Human)

Expression

Mammalian

Mutation

Amino acid 229, Arginine to Glutamine

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-TEV-U1AF37MF77M

Plasmid

#168267

Purpose

Expresses human dmU1A with the F37M/F77M double mutant

Depositor

Joseph Wedekind

Article

Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

Insert

U1A RRM1 crystallization module (SNRPA Human)

Tags

6xHis tag N-terminus, TEV protease cleavage site

Expression

Bacterial

Mutation

changed F37M and F77M

Promoter

T7 promotor

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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