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pSP64-Vasa-GFP

Plasmid

#135595

Purpose

Expression of Vasa-GFP mRNA for microinjection for visualization of Vasa protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Yajima et al Development. 2015 Jun 1;142(11):1960-70.

Insert

Vasa-GFP

Use

In vitro transcription (mrna synthesis)

Tags

EGFP

Promoter

SP6

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC38A7_STOP

Plasmid

#161260

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A7 (SLC38A7 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC38A7_STOP

Plasmid

#161260

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A7 (SLC38A7 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2-H2B-mCherry

Plasmid

#135604

Purpose

Expression of H2B-mCherry mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

H2B-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Promoter

Unknown

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-H2B-mCherry

Plasmid

#135604

Purpose

Expression of H2B-mCherry mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

H2B-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Promoter

Unknown

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hALDOA D34S

Plasmid

#210810

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Tags

HA

Expression

Mammalian

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hALDOA D34S

Plasmid

#210810

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Tags

HA

Expression

Mammalian

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSP64-membrane-mCherry

Plasmid

#135598

Purpose

Expression of membrane-mCherry mRNA for microinjection for visualization of membrane in S. pupuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Promoter

Unknown

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-membrane-mCherry

Plasmid

#135598

Purpose

Expression of membrane-mCherry mRNA for microinjection for visualization of membrane in S. pupuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Promoter

Unknown

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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hSyn1-FLEX-hChR2-tdtomato

Plasmid

#41015

Depositor

Karel Svoboda

Insert

Channelrhodopsin-2

Use

Adenoviral

Tags

tdtomato

Expression

Mammalian

Mutation

codon-optimized (human)

Promoter

synapsin

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
hSyn1-FLEX-hChR2-tdtomato

Plasmid

#41015

Depositor

Karel Svoboda

Insert

Channelrhodopsin-2

Use

Adenoviral

Tags

tdtomato

Expression

Mammalian

Mutation

codon-optimized (human)

Promoter

synapsin

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pLNHA-C1-HsSmaug2_Q

Plasmid

#147285

Purpose

Mammalian Expression of HsSmaug2

Depositor

Elisa Izaurralde

Insert

HsSmaug2 (SAMD4B Human)

Expression

Mammalian

Mutation

one silent mutation compared to the seqeuence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsSmaug2_Q

Plasmid

#147285

Purpose

Mammalian Expression of HsSmaug2

Depositor

Elisa Izaurralde

Insert

HsSmaug2 (SAMD4B Human)

Expression

Mammalian

Mutation

one silent mutation compared to the seqeuence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBP-mTAZ-NFLAG

Plasmid

#31791

Depositor

Michael Yaffe

Article

Hong et al Science. 2005 Aug 12;309(5737):1074-8.

Insert

TAZ (Wwtr1 )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pBP-mTAZ-NFLAG

Plasmid

#31791

Depositor

Michael Yaffe

Article

Hong et al Science. 2005 Aug 12;309(5737):1074-8.

Insert

TAZ (Wwtr1 )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
rat APOBEC1 E63A

Plasmid

#112861

Purpose

plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

APOBEC1 (Apobec1 Rat)

Expression

Mammalian

Mutation

changed Glutamic acid 63 to Alanine

Promoter

CMV promoter

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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rat APOBEC1 E63A

Plasmid

#112861

Purpose

plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

APOBEC1 (Apobec1 Rat)

Expression

Mammalian

Mutation

changed Glutamic acid 63 to Alanine

Promoter

CMV promoter

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-G-Rac1

Plasmid

#180581

Purpose

ddGFP sensor of Rac1

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Rac1

Use

AAV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-G-Rac1

Plasmid

#180581

Purpose

ddGFP sensor of Rac1

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Rac1

Use

AAV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF-EcAvs4

Plasmid

#188806

Purpose

Codon optimized EcAvs4 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs4

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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