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pGAL4-DBD-MCS

Plasmid

#145245

Purpose

Backbone vector expressing GAL4 DNA-binding domain followed by MCS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP18

Plasmid

#22572

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP18 (USP18 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP18

Plasmid

#22572

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP18 (USP18 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-mouse-Caspase-1

Plasmid

#183361

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 (Casp1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

Plasmid

#174081

Purpose

Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

Depositor

Ryan Mehl

Article

Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

Insert

NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0

Use

Tags

NES-Flag

Expression

Mammalian

Mutation

L270G, Y271L, N311G, C313A

Promoter

CMV

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMP71-tCD19-mGFP

Plasmid

#174609

Purpose

expresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

CD19 extracellular and transmembrane domain (Cd19 Mouse)

Use

Tags

palmitoylated EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe10_dTomato_nlsdTomato

Plasmid

#213814

Purpose

AAV construct expressing dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Has Service

AAV PHP.eB

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-PTS1-IRES-Puromycin

Plasmid

#134665

Purpose

Expresses EGFP-tagged peroxisome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

tripeptide SKL (Peroxisome targeting signal 1)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-MLC2(CA)-IRES-GFP

Plasmid

#133928

Purpose

Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

Depositor

Sebastián Dupraz, Frank Bradke

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

EGFP and MLC2(CA) (MYL2 Human)

Use

Tags

Expression

Mammalian

Mutation

MLC2 T18D, S19D

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-Moesin-C-14

Plasmid

#55103

Purpose

Localization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Moesin (MSN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-Moesin-C-14

Plasmid

#55103

Purpose

Localization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Moesin (MSN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
rEcoli XpS

Bacterial Strain

#192872

Purpose

MG1655-C321 -- All 321 UAG codons changed to UAA

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
W118-1_hTGM1-Flag

Plasmid

#180406

Purpose

lentiviral transduction of human TGM1 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM1 (TGM1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pGW-GOI-TurboID

Plasmid

#209378

Purpose

Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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HA-TAZ-S89A

Plasmid

#32840

Depositor

Kunliang Guan

Article

Lei et al Mol Cell Biol. 2008 Apr;28(7):2426-36. E

Insert

TAZ (WWTR1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S89A

Promoter

CMV

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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HA-TAZ-S89A

Plasmid

#32840

Depositor

Kunliang Guan

Article

Lei et al Mol Cell Biol. 2008 Apr;28(7):2426-36. E

Insert

TAZ (WWTR1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S89A

Promoter

CMV

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV: Flag-Akaluc_SV40:PuroR

Plasmid

#183048

Purpose

Expresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

AkaLuc

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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TRUPATH

Plasmid Kit

#1000000163

Purpose

A collection of 20 plasmids encoding alpha, beta, or gamma subunits of the heterotrimeric G protein complex for detection of heterotrimer dissociation. GPCR

Depositor

Bryan Roth

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only