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pKM496 (deletions) Zeo

Plasmid

#109301

Purpose

ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDi-P2A-mCherry

Plasmid

#204358

Purpose

AAV vector for miniDi expression under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDi-P2A-mCherry

Use

AAV

Tags

mCherry

Mutation

The third intracellular loop (ICL3) of hM4Di was …

Promoter

hSyn

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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IKK-2 S177E S181E

Plasmid

#11105

Purpose

Mammalian expression of constitutively active human IKK-2

Depositor

Anjana Rao

Article

Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Insert

IKK-2 (IKBKB Human)

Tags

FLAG

Expression

Mammalian

Mutation

S177E and S181E, constitutively active

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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TAp63alpha-FLAG

Plasmid

#27008

Depositor

David Sidransky

Insert

TAp63alpha (TP63 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 Neo sgTREX1

Plasmid

#127645

Purpose

Knock-out of human TREX1 with NeoR

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

TREX1 sgRNA (TREX1 Human)

Use

Lentiviral

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV.MaCPNS1

Plasmid

#185136

Purpose

non-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

Insert

Synthetic construct isolate AAV-MaCPNS1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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hCRISPRa-v2, genome-wide, top 5 sgRNAs/gene

Pooled Library

#83978

Purpose

Version 2 of the human CRISPRa genome wide library

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Use

Lentiviral

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only
AbVec2.0-IGHG1

Plasmid

#80795

Purpose

Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

Depositor

Hedda Wardemann

Article

Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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Yes-EGFP

Plasmid

#110497

Purpose

Fluorescent Yes

Depositor

Bernardo Mainou

Insert

Yes (YES1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB505

Plasmid

#74847

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe RFP1-KRT5 hygro

Plasmid

#58493

Purpose

mammalian expression of mRFP1-tagged human KRT5

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

KRT5 (KRT5 Human)

Tags

mRFP1

Expression

Mammalian

Available Since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only
4xmts-mNeonGreen

Plasmid

#98876

Purpose

In vivo visualization of mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

4xmts

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET-22b DT 51E/148K

Plasmid

#11081

Depositor

John Collier

Insert

DT

Tags

His

Expression

Bacterial

Mutation

51E/148K, catalytically inactive

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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EF.CMV.RFP

Plasmid

#17619

Depositor

Linzhao Cheng

Article

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (AAV1)

Viral Prep

#100848-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (#100848). In addition to the viral particles, you will also receive purified pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Promoter

Syn

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only
L4552 x6NFATRE (NFAT) Ben DsRed-Express2 in TUPV1

Plasmid

#244201

Purpose

Expression of a DsRed-Express2 reporter under an NFAT-responsive promoter

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

NFAT-responsive promoter (6 NFAT binding sites + YB_TATA), dsRed-Express2 reporter

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS2

Plasmid

#81037

Purpose

HO endonuclease expression

Depositor

John McCusker

Insert

HYGMX

Expression

Yeast

Promoter

TEF

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe4_dTomato_nlsdTomato (AAV PHP.eB)

Viral Prep

#213944-PHPeB

Purpose

Ready-to-use AAV PHP.eB particles produced from pAAV_BiSSTe4_dTomato_nlsdTomato (#213944). In addition to the viral particles, you will also receive purified pAAV_BiSSTe4_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the SST interneuron-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Tags

dTomato

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only
pCMV-PuroR-T2A-eGFP

Plasmid

#234734

Purpose

Puromycin resistance/eGFP cassette in mammalian cells (transfection marker)

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PuroR-T2A-eGFP

Expression

Mammalian

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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FAS promoter luciferase

Plasmid

#8890

Depositor

Bruce Spiegelman

Article

Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

Insert

Fatty Acid Synthetase Promoter (Fasn unknown - rat or mouse, Rat)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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