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MTK2_023

Plasmid

#123715

Purpose

Encodes pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

Plasmid

#86421

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

Depositor

Sue Jaspersen

Publication

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-KAR2SS-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MSCV-JAK3(M511)_IRES_GFP

Plasmid

#206290

Purpose

Expression of mutant JAK3 (M511I) that is able to drive constitutive activation of the JAK/STAT pathway

Depositor

Charles de Bock

Publication

Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

Insert

JAK3 (M511I)

Use

Retroviral

Expression

Mammalian

Mutation

Methionine 511 to Isoleucine

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3_HA_Csx30

Plasmid

#197610

Purpose

Expresses a HA-tagged version of Csx30

Depositor

Feng Zhang

Publication

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

HA-tagged Csx30

Expression

Mammalian

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET-S9

Plasmid

#132948

Purpose

Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

Depositor

James Williamson

Publication

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S9 (rpsI E.coli)

Tags

N-terminal 6xHis-tag

Expression

Bacterial

Mutation

E52C

Promoter

T7

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-SENP3-C526A

Plasmid

#115522

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Publication

Kuo et al Cell Cycle. 2008 Nov 1;7(21):3378-87. do

Insert

SENP3-C526A (Senp3 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

C526A

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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psiCheck-2-3 x mir-34

Plasmid

#40831

Purpose

Luciferase reporter

Depositor

Phillip Zamore

Publication

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

three sites partially complementary to miR-34 (mir-34 Fly)

Use

RNAi

Tags

luciferase

Expression

Insect

Promoter

SV40

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only
pAFM-NbrWT

Plasmid

#40830

Purpose

Expresses Flag, Myc tagged Nbr in Drosophila cells

Depositor

Phillip Zamore

Publication

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

Nbr (Nbr Fly)

Tags

3xFLAG and 6xMyc

Expression

Insect

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
polyQ74-GFP

Plasmid

#231893

Purpose

Forms cytosolic HTT partial exon 1 Q74 aggregates

Depositor

Lukas Kapitein

Publication

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74 (HTT Human)

Tags

EGFP

Expression

Mammalian

Mutation

CAG repeat expansion

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5B-Rigor

Plasmid

#172204

Purpose

Expresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Publication

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF5B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Tet-on Advanced -BGH-PA

Plasmid

#114379

Purpose

Express reverse tetracycline inducible rtTA transcriptional activator

Depositor

Yuet Wai Kan

Publication

Ye et al Proc Natl Acad Sci U S A. 2010 Nov 9;107

Insert

rtTA and BGH-pA

Expression

Mammalian

Promoter

CAG

Available since

Dec. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMito-mCherry-FRB_IRES_FKBP(III)-GBPen

Plasmid

#128268

Purpose

Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 3x FKBP-GBPenhancer (GFP Nanobody with 3 FKBP domains).

Depositor

Stephen Royle

Publication

Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

Inserts

MitoTrap
FKBP(III)-GBPen

Expression

Mammalian

Promoter

CMV and IRES

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCFD3-frame_selector_2

Plasmid

#127555

Purpose

Drosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Publication

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 2

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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+36GFP-LPETG

Plasmid

#71754

Purpose

+36GFP-LPETG for expression in E.Coli

Depositor

David Liu

Publication

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

(GGS)9_(pos36GFP)_6XHis_LPETG

Tags

6XHis and Sortase (LPETG)

Expression

Bacterial

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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mVenus-PA-MKI

Plasmid

#65457

Purpose

photoactivatable myosin light-chain kinase inihibition

Depositor

Klaus Hahn

Publication

Yi et al ACS Synth Biol. 2014 Nov 21;3(11):788-95

Insert

MKI

Expression

Mammalian

Promoter

CMV

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-GP-2-ATRX ADD

Plasmid

#59698

Purpose

Contains histone modification interacting domain (ATRX ADD)

Depositor

Albert Jeltsch

Publication

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

ATRX ADD (ATRX Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TET-pLKO.1 PURO shSMAD4 #1

Plasmid

#83089

Purpose

Lentiviral shRNA vector for inducible knockdown of human SMAD4 (cross reacts with mouse Smad4)

Depositor

Kevin Janes

Publication

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shSMAD4 (SMAD4, Smad4 Human, Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pTRE2-Bla(HA–RILP)

Plasmid

#102425

Purpose

HA tag fused to the N-terminus of RILP for the expression in mammalian cells.

Depositor

Steven Weinman

Publication

Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

Rab interacting lysosomal protein (RILP Human)

Tags

HA

Expression

Mammalian

Promoter

Tet-responsive

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFPL-Rg

Plasmid

#61654

Purpose

Gateway vector for generating red fluorescent protein fusions in fungi

Depositor

Mark Farman

Publication

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

monomeric red fluorescent protein

Expression

Plant

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT7-agrBD-I

Plasmid

#53439

Purpose

T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

Depositor

Cynthia Collins

Publication

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrBD locus

Use

Synthetic Biology

Tags

V5 epitope, 6x HIS (not expressed on agrB or agrD…

Expression

Bacterial

Mutation

none

Promoter

T7-lac

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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