We narrowed to 17,077 results for: Por

1. P2A-mCherry-EhT backbone

   Plasmid

   #215549

   Purpose

   An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomato

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRXG

   Plasmid

   #113643

   Purpose

   This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

   Depositor

   Jeffrey Barrick

   Article

   Monk et al ACS Synth Biol. 2016 Sep 20.

   Insert

   mRFP1-linker-sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-epNTR

   Plasmid

   #62213

   Purpose

   epNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2

   Depositor

   Harold Burgess

   Article

   Tabor et al J Neurophysiol. 2014 Aug 15;112(4):834-4

   Insert

   epNTR
   

   Use

   Mrna synthesis

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. P336-FBP_11

   Plasmid

   #139702

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-GfaABC1D-GRAB_g5-HT3.0

   Plasmid

   #208727

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytes

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   GfaABC1D

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. T2A 3xNLS GFP

   Plasmid

   #227721

   Purpose

   Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Oliver Hobert, Haosheng Sun

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AzoRS-4

   Plasmid

   #182884

   Purpose

   A pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

   Depositor

   Miriam Amiram

   Article

   Israeli et al Adv. Funct. Mater. 2021, 31, 2011276

   Insert

   AzoRS-4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-28a(+)::NL

   Plasmid

   #141289

   Purpose

   NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   NLUC
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTK-RetE1-Citrine

   Plasmid

   #127779

   Purpose

   Enhancer reporter construct containing the chicken RetE1 enhancer driving Citrine activity

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   RetE1
   

   Use

   Enhancer reporter construct

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3XFLAG-NSarQ27G-VMAn-FKBP

    Plasmid

    #70229

    Purpose

    Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (N-terminal portion)
    

    Use

    Tags

    3XFLAG, FK506- and rapamycin-binding protein (FKB…

    Expression

    Mammalian

    Mutation

    Changed Gln 27 to Gly

    Promoter

    CMV

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pLentiPGK Puro DEST JNKKTRrsGreenF-E

    Plasmid

    #205764

    Purpose

    Expression of JNK KTR rsGreenF-E under PKG promoter (With Puromycin Resistance)

    Depositor

    Edward Boyden

    Article

    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

    Insert

    JNK KTR rsGreenF-E
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCMV Puro DEST ERKKTRrsFastLime

    Plasmid

    #205765

    Purpose

    Expression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)

    Depositor

    Edward Boyden

    Article

    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

    Insert

    ERK KTR rsFastLime
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFH2.155_LV-Puro-T2A-mTagBFP2

    Plasmid

    #205553

    Purpose

    Express puromycin resistance marker and mTagBFP2

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Puro-T2A-mTagBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS1NB c Rosella I

    Plasmid

    #71245

    Purpose

    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

    Depositor

    Mark Prescott

    Article

    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

    Insert

    Rosella
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE-Medium-FT

    Plasmid

    #31914

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Medium-FT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

    Promoter

    PTight Tet-responsive PminCMVd

    Available Since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-QFGal4-SV40pA

    Plasmid

    #155118

    Purpose

    Middle entry vector containing QFGal4

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QFGal4
    

    Use

    Gateway middle entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-DIO-GRAB_g5-HT3.0

    Plasmid

    #208726

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PCDNA3-GFP1-9 T2A mCherry

    Plasmid

    #124430

    Purpose

    Expresses GFP1-9 and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    GFP1-9 T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMedium-FT-N1

    Plasmid

    #31911

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Medium-FT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

    Promoter

    CMV

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

    Plasmid

    #214995

    Purpose

    Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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