We narrowed to 17,323 results for: Por

1. CpxR-GFP

   Plasmid

   #220150

   Purpose

   To track the natural CpxR promoter's transcriptional dynamics

   Depositor

   Andre Sanches Ribeiro

   Article

   Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

   Insert

   CpxR promoter only
   

   Expression

   Bacterial

   Available Since

   Aug. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCdrA-gfpC

   Plasmid

   #111614

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLD-puro-MTS-EGFP

   Plasmid

   #190270

   Purpose

   Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

   Depositor

   Christian Münch

   Article

   Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

   Insert

   MTS-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Tight TRE promoter

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBDC

   Plasmid

   #135188

   Purpose

   Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   CmR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Promoter

   cat

   Available Since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUB-GFP

   Plasmid

   #11155

   Purpose

   Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   Ubiquitin C promoter (UBC Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

   Plasmid

   #205550

   Purpose

   Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN24-MCP
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP

   Plasmid

   #213119

   Purpose

   Expression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   ActA(1-584)-BFP-FKBP (actA Listeria monocytogenes)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. P362-FBP_12

   Plasmid

   #139703

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mKO2-N1

   Plasmid

   #54625

   Purpose

   Localization: N1 Cloning Vector, Excitation: 551, Emission: 565

   Depositor

   Michael Davidson, Atsushi Miyawaki

   Article

   Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

   Type

   Empty backbone

   Tags

   mKO2

   Expression

   Mammalian

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE-Fast-FT

    Plasmid

    #31913

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Fast-FT
    

    Expression

    Mammalian

    Mutation

    M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

    Promoter

    PTight Tet-responsive PminCMVd

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-EF1a-MinD-IRES-Puro

    Plasmid

    #213114

    Purpose

    Expression of untagged MinD in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF-BFP

    Plasmid

    #138272

    Purpose

    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    BFP H66 PAM
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    H66, PAM motif

    Promoter

    Ef1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNCS-mClover3

    Plasmid

    #74236

    Purpose

    Constitutive expression of mClover3 in bacteria

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Has Service

    Cloning Grade DNA

    Insert

    mClover3
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCXLE-dCas9VP192-T2A-EGFP-shP53

    Plasmid

    #69535

    Purpose

    Episomal plasmid encoding dCas9VP192 and p53 shRNA

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    dCas9-dCas9VP192-GFP-shp53
    

    Use

    CRISPR

    Tags

    EGFP

    Promoter

    CAG

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE-Slow-FT

    Plasmid

    #31915

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Slow-FT
    

    Expression

    Mammalian

    Mutation

    M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

    Promoter

    PTight Tet-responsive PminCMVd

    Available Since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS1NB c Rosella I

    Plasmid

    #71245

    Purpose

    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

    Depositor

    Mark Prescott

    Article

    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

    Insert

    Rosella
    

    Expression

    Yeast

    Promoter

    PGK1

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTXTL-T7max-FLuc

    Plasmid

    #188311

    Purpose

    Expression of Firefly luciferase via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    Firefly luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Promoter

    T7Max

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3-Neo-EF1a-GFP

    Plasmid

    #69134

    Purpose

    Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

    Depositor

    Martin Bonamino

    Article

    Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

    Type

    Empty backbone

    Use

    Sleeping beauty transposon

    Expression

    Mammalian

    Promoter

    U3MSCV

    Available Since

    Jan. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

    Plasmid

    #45574

    Purpose

    Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    co I-Sce I
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFREE_Zeo

    Plasmid

    #92053

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Promoter

    pRhamBAD and ptet

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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