We narrowed to 5,839 results for: pCas

1. sgMTOR_49

   Plasmid

   #125132

   Purpose

   Targeting 49th exon of human MLST8 gene by CRISPR-Cas9

   Depositor

   Jin Chen

   Article

   Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

   Insert

   MTOR (MTOR Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyCas9 PE2-3HA

   Plasmid

   #169853

   Purpose

   Mammalian Expression, SpyCas9 prime editor with 3HA-tag

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SpCas9-H840A-M-MLV-3HA
   

   Tags

   3XHA tag and bpSV40 NLS

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRvCAHS1-mEGFP-NLS

   Plasmid

   #205013

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
   

   Use

   Tardigrade expression

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRvSAHS1-mCherry-NLS

   Plasmid

   #205009

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
   

   Use

   Tardigrade expression

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. HSF1 KO

   Plasmid

   #200207

   Purpose

   gRNA for HSF1 KO

   Depositor

   Sheila Teves

   Article

   Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

   Insert

   HSF1 KO (Hsf1 Mouse)
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sox17(2A-phiC31o)

   Plasmid

   #140762

   Purpose

   Targeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Insert

   phiC31o
   

   Use

   Mouse Targeting

   Promoter

   Endogenous Sox17

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sox1(2A-Cre)

   Plasmid

   #140760

   Purpose

   Targeting vector to Sox1 gene locus for induction of P2A-fused Cre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(WT) sequence.

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Insert

   Cre
   

   Use

   Mouse Targeting

   Promoter

   Endogenous Sox1

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. myc-hRAD18 C207F

   Plasmid

   #68829

   Purpose

   Expresses c-myc-tagged human RAD18 with C207F mutation

   Depositor

   Satoshi Tateishi

   Article

   Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

   Insert

   c-myc tagged human RAD18 with C207F mutation (RAD18 Human)
   

   Tags

   c-myc

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pJT112_GalL_nCDA1-xBE3

   Plasmid

   #141318

   Purpose

   Expressing base editor nCDA1-xBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-xBE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

   Promoter

   GalL

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGB2alpha2 35s:hCas9:tNos (GB0639)

    Plasmid

    #68222

    Purpose

    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    hCas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. ABE8e(TadA-8e K20A R21A)

    Plasmid

    #138496

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e K20A R21A)-nSpCas9
    

    Expression

    Mammalian

    Mutation

    TadA-8e K20A R21A

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. VRER variant

    Plasmid

    #138561

    Purpose

    Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRER
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEPQD1CB0202

    Plasmid

    #185626

    Purpose

    MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-sgRNA_Dicer_3

    Plasmid

    #68809

    Purpose

    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

    Depositor

    Constance Ciaudo

    Article

    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

    Insert

    sgRNA mouse Dicer1
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Px458-Cas9n-Trp63-Exon4-2sgRNA

    Plasmid

    #88849

    Purpose

    CRISPR KO of Trp63

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp63 (Trp63 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

16. AtS/MAR10 insulator sequence (GB3458)

    Plasmid

    #160638

    Purpose

    pEGB3a1 AtS/MAR10

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    AtS/MAR10 insulator sequence
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

    Plasmid

    #159658

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

    Plasmid

    #159657

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-sgRNA_Dicer_2

    Plasmid

    #68808

    Purpose

    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

    Depositor

    Constance Ciaudo

    Article

    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

    Insert

    sgRNA mouse Dicer1
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

    Plasmid

    #160610

    Purpose

    Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:AsCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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