We narrowed to 5,844 results for: pcas

1. pJL1-mRFP1 gRNA

   Plasmid

   #117052

   Purpose

   In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

   Insert

   anti-mRFP1 SpCas9 gRNA
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TAC-OKMS-Cas9

   Plasmid

   #120353

   Purpose

   piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OKMS cassette
   

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Mutation

   Klf4 [10-483]

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330 _HITI donor B6

   Plasmid

   #172847

   Purpose

   DRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   DRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Px458-Cas9n-Trp63-Exon4-1sgRNA

   Plasmid

   #88848

   Purpose

   CRISPR KO of Trp63

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp63 (Trp63 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pC1300_pUB10_pco-nCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

   Plasmid

   #197981

   Purpose

   T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pco-nCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   pUBQ10

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgMTOR_49

   Plasmid

   #125132

   Purpose

   Targeting 49th exon of human MLST8 gene by CRISPR-Cas9

   Depositor

   Jin Chen

   Article

   Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

   Insert

   MTOR (MTOR Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpyCas9 PE2-3HA

   Plasmid

   #169853

   Purpose

   Mammalian Expression, SpyCas9 prime editor with 3HA-tag

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SpCas9-H840A-M-MLV-3HA
   

   Tags

   3XHA tag and bpSV40 NLS

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRvCAHS1-mEGFP-NLS

   Plasmid

   #205013

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
   

   Use

   Tardigrade expression

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRvSAHS1-mCherry-NLS

   Plasmid

   #205009

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
   

   Use

   Tardigrade expression

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. HSF1 KO

    Plasmid

    #200207

    Purpose

    gRNA for HSF1 KO

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    HSF1 KO (Hsf1 Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sox17(2A-phiC31o)

    Plasmid

    #140762

    Purpose

    Targeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.

    Depositor

    Hideyuki Okano

    Article

    Serizawa et al Development. 2019 Nov 4;146(21). pii: de

    Insert

    phiC31o
    

    Use

    Mouse Targeting

    Promoter

    Endogenous Sox17

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Sox1(2A-Cre)

    Plasmid

    #140760

    Purpose

    Targeting vector to Sox1 gene locus for induction of P2A-fused Cre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(WT) sequence.

    Depositor

    Hideyuki Okano

    Article

    Serizawa et al Development. 2019 Nov 4;146(21). pii: de

    Insert

    Cre
    

    Use

    Mouse Targeting

    Promoter

    Endogenous Sox1

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. myc-hRAD18 C207F

    Plasmid

    #68829

    Purpose

    Expresses c-myc-tagged human RAD18 with C207F mutation

    Depositor

    Satoshi Tateishi

    Article

    Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

    Insert

    c-myc tagged human RAD18 with C207F mutation (RAD18 Human)
    

    Tags

    c-myc

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pJT112_GalL_nCDA1-xBE3

    Plasmid

    #141318

    Purpose

    Expressing base editor nCDA1-xBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-xBE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

    Promoter

    GalL

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGB2alpha2 35s:hCas9:tNos (GB0639)

    Plasmid

    #68222

    Purpose

    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    hCas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. ABE8e(TadA-8e K20A R21A)

    Plasmid

    #138496

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e K20A R21A)-nSpCas9
    

    Expression

    Mammalian

    Mutation

    TadA-8e K20A R21A

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. VRER variant

    Plasmid

    #138561

    Purpose

    Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRER
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEPQD1CB0202

    Plasmid

    #185626

    Purpose

    MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-sgRNA_Dicer_3

    Plasmid

    #68809

    Purpose

    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

    Depositor

    Constance Ciaudo

    Article

    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

    Insert

    sgRNA mouse Dicer1
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Px458-Cas9n-Trp63-Exon4-2sgRNA

    Plasmid

    #88849

    Purpose

    CRISPR KO of Trp63

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp63 (Trp63 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only