We narrowed to 5,907 results for: SUP

1. pDule2-pCNF

   Plasmid

   #85495

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSR358.4

   Plasmid

   #125114

   Purpose

   Expression of narL(1-159aa)-liaR(154-211aa) chimera under PLtetO-1, output Promoter PliaI121

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   narL(REC)-liaR(DBD)159
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. GALT-SEP-mRuby3

   Plasmid

   #200938

   Purpose

   trans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Inserts

   GalT (B4GALT1 Human)
   SEP-mRuby3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MGAT-SEP-mRuby3

   Plasmid

   #200937

   Purpose

   cis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Inserts

   MGAT2 (MGAT2 Human)
   SEP-mRuby3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TfR-sfGFP-myc tag-SpyCatcher003

   Plasmid

   #133451

   Purpose

   Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   TfR-sfGFP-myc tag-SpyCatcher003
   

   Use

   Tags

   GSSGS and myc tag

   Expression

   Mammalian

   Mutation

   Contains C20 and A23 mutations that improve plasm…

   Promoter

   CMV

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJL1

   Plasmid

   #69496

   Purpose

   pJL1 expression vector with sfGFP

   Depositor

   Michael Jewett

   Article

   Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

   Insert

   Super folder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDule-pCNF

   Plasmid

   #85494

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS_4xMma PylT sfGPF 150TAG

   Plasmid

   #154766

   Purpose

   plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG in GFP reporter, U25C in PylT

   Promoter

   EF1

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_4xMma PylT_GFP 150TAG 6xHis

   Plasmid

   #140015

   Purpose

   Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   150TAG

   Promoter

   EF1

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDule2-Mb haloTyrRS C6

    Plasmid

    #160378

    Purpose

    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

    Depositor

    Ryan Mehl

    Article

    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

    Insert

    C6 HaloTyrosine tRNA synthatase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    L270S, Y271L, N311G, C313T

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS_4xMma PylT_FLAG-Mma PylRS

    Plasmid

    #140009

    Purpose

    Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_4xMx PylT_GFP 150TAG

    Plasmid

    #140016

    Purpose

    Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG, K46E (Please see depositor comments below)

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpyDock

    Plasmid

    #124618

    Purpose

    Expresses SpyDock to bind SpyTag non-covalently for affinity purification

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyDock
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E77A mutation prevents isopeptide bond formation,…

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRMC2-msfGFP

    Plasmid

    #194913

    Purpose

    Tetracycline inducible expression of msfGFP in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno Sequence followed by monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. LAMP1-SEP-mRuby3

    Plasmid

    #200939

    Purpose

    endolysosome localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Inserts

    LAMP1 (LAMP1 Human)
    SEP-mRuby3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX304-VHL-IRES-GFP

    Plasmid

    #187641

    Purpose

    VHL Expression vector with GFP

    Depositor

    William Kaelin

    Article

    Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

    Insert

    VHL (VHL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX307-VHL(C162F)-3F-BirA

    Plasmid

    #220149

    Purpose

    Expresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    von Hippel-Lindau tumor suppressor (VHL Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Mutation

    changed cysteine 162 to phenylalanine; codon opti…

    Promoter

    EF-1a

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pExpreS2-1-GFP

    Plasmid

    #175363

    Purpose

    ZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cells

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Insert

    sgGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    F64L, S65C, and I167T

    Promoter

    Fused Actin-HSP70

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP C3-Lats1

    Plasmid

    #19053

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Large tumor suppressor kinase 1, N mutant (LATS1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    **

    Promoter

    Available since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

    Plasmid

    #174890

    Purpose

    Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    U25C PylT

    Promoter

    EF1

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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