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pLM033

Plasmid

#134245

Purpose

Retroviral plasmid with H4 EGFP insert

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

Human H4 + eGFP/HALO retroviral tagging (HRH4 Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3 T2P 800

Plasmid

#131147

Purpose

To investigate activity of a 800 bp region upstream of mouse Trib2.

Depositor

Karen Keeshan

Article

Rishi et al Blood. 2014 Apr 10;123(15):2389-400. doi

Insert

-800 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)

Tags

luciferase

Expression

Mammalian

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3 T2P 2.6

Plasmid

#131151

Purpose

To investigate activity of a 2.6 kb region upstream of mouse Trib2.

Depositor

Karen Keeshan

Article

Rishi et al Blood. 2014 Apr 10;123(15):2389-400. doi

Insert

-2.6kb - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)

Tags

luciferase

Expression

Mammalian

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-RIP2_3K

Plasmid

#131205

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 K209/410/538R (RIPK2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

K209/410/538R

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3 T2P 500

Plasmid

#131150

Purpose

To investigate activity of a 500 bp region upstream of mouse Trib2.

Depositor

Karen Keeshan

Article

Rishi et al Blood. 2014 Apr 10;123(15):2389-400. doi

Insert

-500 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)

Tags

luciferase

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD-H253A-R256A-R260A

Plasmid

#127788

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in His2…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD-H253A-R256A-R260A-R296A

Plasmid

#127789

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in His2…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD-R385A-K386A-K389A-K390A

Plasmid

#127792

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in Arg3…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-NBR1(F562A/Y731A/ΔUBD)

Plasmid

#119692

Purpose

For mammalian expression of LIR double mutant NBR1 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

NBR1 (NBR1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

F562A/Y731A & UBD deletion (1-906 ), See depo…

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN iRFP670-OPTN(F178A/D474N)

Plasmid

#119694

Purpose

For mammalian expression of iRFP-tagged LIR/UBD double mutant OPTN

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

OPTN (OPTN Human)

Use

Retroviral

Tags

iRFP670

Expression

Mammalian

Mutation

F178A/D474N

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pJL15

Plasmid

#117430

Purpose

Expression of ARL2

Depositor

Richard Kahn

Article

Clark et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

ARL2 (ARL2 Human)

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_4xT790_28bp

Plasmid

#114720

Purpose

4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_T790_28bp

Plasmid

#114722

Purpose

28 bp fragment of the WT EGFR allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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PAK4-3'UTR-BS2

Plasmid

#113091

Purpose

Luciferase reporter containing PAK4 3' UTR with the second predicted MiR-199a-3p binding

Depositor

James Donahue

Article

Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

Insert

p21 Activated kinase 4 (PAK4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

none

Promoter

PGK

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PAK4-3'UTR-BS1

Plasmid

#113090

Purpose

Luciferase reporter containing PAK4 3' UTR with the first predicted MiR-199a-3p binding site

Depositor

James Donahue

Article

Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

Insert

p21 Activated kinase 4 (PAK4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

none

Promoter

PGK

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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NET39 Short pmRFP-N2 (451)

Plasmid

#61981

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET39 (PPAPDC3 Human)

Tags

mRFP

Expression

Mammalian

Mutation

Short form of NET39

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-PIM2mut3

Plasmid

#53742

Purpose

promoter region of PIM2 lacking the 2 forkhead binding sites

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Mutation

deletion of 2 forkhead binding sites (bp# 395-497…

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA_MET_D14_3Flag

Plasmid

#182495

Purpose

Contains cDNA of the MET gene without the exon 14

Depositor

Paolo Comoglio

Article

Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

Insert

MET (MET Human)

Tags

C-terminal 3xFlag tag

Expression

Mammalian

Mutation

Exon 14 deleted

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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LAMP1-RpHLuorin2

Plasmid

#171720

Purpose

Lysosomal expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.cTNT.iCre

Plasmid

#69916

Purpose

Used to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.

Depositor

William Pu

Article

Lin et al Circ Res. 2015 Jan 2;116(1):35-45. doi:

Has Service

AAV9

Insert

improved cre (iCre) recombinase and tdTomato

Use

AAV

Tags

The iCre coding frame and tdTomato coding frame i…

Promoter

Chicken cardiac troponin T

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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