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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,726 results for: EMB

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Showing: 5341 - 5360 of 11726 results

  2. pAL725

    Plasmid
    #119862
    Purpose
    precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
    Tags
    none
    Expression
    Bacterial
    Mutation
    Leucine 244 to Cysteine, Aspartate 287 to Cysteine
    Promoter
    T5
    Available Since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119862-plasmid-map-sequence-id-232032

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  3. ABCB8 (ABCB8A-c016)

    Plasmid
    #110266
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ABCB8 (ABCB8 Human)
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110266-plasmid-map-sequence-id-212276

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  4. pCAG-B2UP49_GFP

    Plasmid
    #103071
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    CMV
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103071-plasmid-map-sequence-id-201896

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  10. pChlr-1SE

    Plasmid
    #52140
    Purpose
    Encodes module 1 with amino acids 12S-16E for recognition of nt 8G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 16Q to 16E in Module 1
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid
    #222099
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Vitiosangium-bGSDM-thrombin ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222099-plasmid-map-sequence-id-437094

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Showing: 5341 - 5360 of 11726 results