We narrowed to 11,022 results for: ENA;

1. SpIMPDH

   Plasmid

   #206980

   Purpose

   Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

   Depositor

   Vern Schramm

   Article

   Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

   Insert

   SpIMPDH (guaB Streptococcus pyogenes)
   

   Use

   Tags

   His tag (with TEV cleavage site)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyCas9 PE2*-3HA

   Plasmid

   #169854

   Purpose

   Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SpCas9-H840A-star-M-MLV-3HA
   

   Use

   Tags

   3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. DNAJC11-EGFP

   Plasmid

   #210368

   Purpose

   Expresses DNAJC11 fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   DNAJC11 (DNAJC11 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-RFP-IKZF3-sh3

   Plasmid

   #69044

   Purpose

   3rd generation lentiviral vector expressing IKZF3 shRNA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF3 (IKZF3 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. LSB-hsa-miR-7-5p

   Plasmid

   #103723

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-7-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-7-5p target
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GW1-mito-mRuby2-roGFP1

   Plasmid

   #107219

   Purpose

   Mammalian expression plasmid to target the rmRuby1-roGFP1 sensor to the mitochondrial matrix.

   Depositor

   Mathew Tantama

   Article

   Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

   Insert

   mRuby2-roGFP1
   

   Use

   Tags

   4xCox8

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. FastLightR-bRaf-mVenus

   Plasmid

   #162155

   Purpose

   Expresses FastLightR-bRaf-mVenus in mammalian cells

   Depositor

   Andrei Karginov

   Article

   Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

   Insert

   FastLightR-bRaf-mVenus
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Valine 600 is substituted with Glutamic acid

   Promoter

   CMV

   Available Since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. plenti-UbcP-Myc-CRBN-pGK-HYG

   Plasmid

   #107376

   Purpose

   Lentiviral expression of myc-CRBN

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   UbcP

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. tet H-ras val12

   Plasmid

   #8900

   Depositor

   Lynda Chin

   Article

   Chin et al Nature. 1999 Jul 29. 400(6743):468-72.

   Insert

   ras val12 (HRAS Human)
   

   Use

   Tags

   Tet-op promoter

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available Since

   Jan. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pLenti-TRE3G-BE3-PGK-Puro

    Plasmid

    #110845

    Purpose

    Lentiviral vector for dox-inducible expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    TRE3G

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124542

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MDH1-miR-221-PGK-GFP-Blast

    Plasmid

    #31519

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-221 (Mir221 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pEGFP-N2-RIPK1 3KR

    Plasmid

    #78847

    Purpose

    To detect localization of RIPK1 3KR protein in live mammalian cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K571R, K604R, K627R

    Promoter

    CMV

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

14. HDM_omicron_B11529_IDTDNA

    Plasmid

    #204142

    Purpose

    Expression plasmid for SARS-CoV-2 BA.1 variant spike

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    SARS-CoV-2 BA.1 variant spike (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    21 amino acid cytoplasmic tail deletion

    Promoter

    CMV

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-FDIO-IGK-dAPEX2-KDEL

    Plasmid

    #117184

    Purpose

    Plasmid name in publication: pAAV-FDIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    IGK-dAPEX2-KDEL
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEMHE-NLS-Beta-catenin-S33Y

    Plasmid

    #138537

    Purpose

    For making Beta-catenin-S33Y RNA

    Depositor

    Carmen Williams

    Article

    Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

    Insert

    Beta-catenin
    

    Use

    In vitro transcription

    Tags

    2XFLAG and 2xNLS

    Expression

    Mutation

    changed serine 33 to tyrosine

    Promoter

    T7

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. NSP14-Flag

    Plasmid

    #210346

    Purpose

    Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP14 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-HA-TRAIL-R4

    Plasmid

    #61385

    Purpose

    Expresses HA tagged TRAIL receptor 4

    Depositor

    Francis Chan

    Article

    Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

    Insert

    TRAIL receptor 4 (TNFRSF10D Human)
    

    Use

    Tags

    HA and TNF receptor leader peptide

    Expression

    Mammalian

    Mutation

    TRAIL-R4 leader peptide (aa1-29) replaced with TN…

    Promoter

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

19. Polymerase expression - pCMV-Phi29 (LM2708)

    Plasmid

    #208965

    Purpose

    Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

    Plasmid

    #119067

    Purpose

    Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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