We narrowed to 5,488 results for: PID;

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pSUMO ACD11

Plasmid

#170738

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

Insert

acd11 (ACD11 Mustard Weed)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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EGFR D770-N771 insNPG

Plasmid

#11016

Purpose

Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D770-N771 insNPG (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D770-N771 insNPG

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pET32a-hEGF

Plasmid

#199231

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Epidermal growth factor, partial (EGF Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE-BI-TRIM21

Plasmid

#207838

Purpose

Inducible TRIM21 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Article

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

TRIM21 (TRIM21 Human)
mCherry
hIgG1-FC
VHH-GFP4

Tags

weak NLS

Expression

Mammalian

Mutation

All K mutated to R

Promoter

TRE3G BI promoter

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pNK5712

Plasmid

#219752

Purpose

pGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - nnH3H_v2 - tAOX

Expression

Yeast

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE-BI-osTIR1

Plasmid

#207840

Purpose

Inducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Article

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

osTIR
mCherry
mAID_-VHH-GFP4

Tags

3X HA-Tag and weak NLS

Expression

Mammalian

Mutation

All K mutated to R

Promoter

TRE3G BI promoter

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pNK5867

Plasmid

#219751

Purpose

pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - mcitHispS - tAOX

Expression

Yeast

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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mEGFP-PI35P2

Plasmid

#92419

Purpose

Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

Depositor

Geert van den Bogaart

Article

Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

Insert

MCOLN1 (Mcoln1 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Synthetic gene, codon optimized for human.

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pX028

Plasmid

#167151

Purpose

MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnHispS-ocsT

Expression

Plant

Promoter

p35s

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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EGFR (del3) L747-E749del, A750P

Plasmid

#11015

Purpose

Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (del3) (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L747-E749 deleted, A750P

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pX029

Plasmid

#167152

Purpose

MoClo-compatible Level 1 (position 3) vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnH3H-ocsT

Expression

Plant

Promoter

p35s

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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PAmCherry-N1-FRB-Tubbyc

Plasmid

#202678

Purpose

Photoactivatable and rapamycin recruitable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

Mus musculus Tub (243-505):MTOR(2021-2113):PAmCherry1 (Tub, Mtor Mouse)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE-BI-aaAFB2

Plasmid

#207839

Purpose

Inducible AFB2 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Article

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

atAFB2
mCherry
miniIAA7
VHH-GFP4

Tags

3X FLAG-Tag and weak NLS

Expression

Mammalian

Mutation

All K mutated to R

Promoter

TRE3G BI promoter

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTRF.1 udsVenus (P_JUND)

Plasmid

#58692

Purpose

A rapidly responsive reporter of endogenous JUND promoter activity (Lentiviral expression vector for reporter ultradestabilized Venus)

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

endogenous promoter of JUND (JUND Human)

Use

Lentiviral

Tags

udsVenus

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only
pNK3203

Plasmid

#219749

Purpose

DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pCMV - mcitHispS - tSV40

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2

Plasmid

#212112

Purpose

Expression of C-terminally tagged GABARAP(G116A) with mTagBFP2

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

GABARAP(G116A)-mTagBFP2 (GABARAP Entacmaea quadricolor, Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of GABARAP

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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Raichu-OsRac1 CA

Plasmid

#133264

Purpose

Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1(G19V) (LOC4325879 Oryza sativa)

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

OsRac1 G19V

Promoter

Ubi

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Raichu-OsRac1 DN

Plasmid

#133263

Purpose

Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1(T24N) (LOC4325879 Oryza sativa)

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

OsRac1 T24N

Promoter

Ubi

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pSUMO CPTP

Plasmid

#170735

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

CPTP (CPTP Human)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-hMSN

Plasmid

#211823

Purpose

Express human MSN in mammalian cells

Depositor

Haian Fu

Article

Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

Insert

MSN (MSN Human)

Tags

GST tag

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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