167,318 results

1. pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

   Plasmid

   #134868

   Purpose

   Expresses EGFP-tagged lysosomal marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lysosomal-associated membrane protein 1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cav-RA

   Plasmid

   #209881

   Purpose

   Dimerization dependent fluorescent protein RA anchored to cytosolic face of the caveolae with mouse CAVIN1

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddRFP A
   

   Tags

   CAVIN1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG-FR-GECO1c

   Plasmid

   #163682

   Purpose

   Far-red fluorescent calcium indicator FR-GECO1c

   Depositor

   Robert Campbell

   Article

   Dalangin et al bioRxiv 2020.11.12.380089

   Insert

   FR-GECO1c
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

   Plasmid

   #211687

   Purpose

   U6-sgRNA-EFS-Cas9-P2A-Puro

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   SpyCas9 and guide RNA recipient vector
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmEGFP-C1-Nir3-LNS2

   Plasmid

   #220087

   Purpose

   C-terminal PA binding domain of Nir3

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PITPNM2 (PITPNM2 Human)
   

   Tags

   mEGFP-GGGGSHM

   Expression

   Mammalian

   Mutation

   amino acids 925-1209

   Promoter

   CMV

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET22b RNase A

   Plasmid

   #58903

   Purpose

   Bacterial expression of bovine pancreatic ribonuclease A

   Depositor

   Ronald Raines

   Article

   delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

   Insert

   RNase A (RNASE1 Bovine)
   

   Tags

   pelB sequence

   Expression

   Bacterial

   Mutation

   See Depositor Comments below

   Promoter

   T7

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV1)

   Viral Prep

   #87306-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Li Zhang

   Article

   Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

   Promoter

   EF1a

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

8. qTAG-C-mStayGold-Blast

   Plasmid

   #227254

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol2-UAS-zmiRFP

   Plasmid

   #108415

   Purpose

   Codon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   zmiRFP
   

   Use

   Zebrafish

   Promoter

   UAS

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. MARS_pNIC-CTB10H

    Plasmid

    #153053

    Purpose

    Expression construct for producing Avi tagged Methionyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    MARS (MARS1 Human)
    

    Tags

    TEV-Avi-His10

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. LV-hGFAP-SOX2-IRES-GFP

    Plasmid

    #183909

    Purpose

    Expression of human SOX2 and GFP under the human GFAP ABC1D promoter

    Depositor

    Chun-Li Zhang

    Insert

    SOX2 (GFAP, SOX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT3-EF1a-c-Met

    Plasmid

    #31784

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    Met (MET Human)
    

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-SKII-3XHA-fCLIP-NAT

    Plasmid

    #188921

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-fCLIP-NAT
    

    Expression

    Yeast

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV5)

    Viral Prep

    #26975-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    CaMKII

    Tags

    mCherry

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pQE30-His-NDK

    Plasmid

    #124136

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Nucleotide diphosphate kinase (ndk Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_479

    Plasmid

    #179099

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    ABE8e (SpG)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. IDG_CACNG3_OE_1

    Plasmid

    #161622

    Purpose

    Overexpresses CACNG3

    Depositor

    Mike McManus

    Insert

    CACNG3 (CACNG3 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-cGAS-HA

    Plasmid

    #231895

    Purpose

    Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    cGAS (CGAS Human)
    

    Tags

    3xHA and mCherry

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC1-HyPer-Red-C199S

    Plasmid

    #48252

    Purpose

    Hydrogen peroxide insensitive version of HyPerRed to be used as a pH control in HyPer experiments.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HYPER-RED-C199S
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. rGluA2 (px330:2-guideCas9)

    Plasmid

    #169439

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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