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pET22b-ecDHFR-N23PP/G51PEKN

Plasmid

#118583

Purpose

Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

N23 mutated to P23, G51 mutated to P51, a Pro res…

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4C (216-233) (pGEX2T(TEV))

Plasmid

#80635

Purpose

Residues 216-233 of CHMP4C; Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4C (CHMP4C Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 216-233

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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ADAP1

Plasmid

#163905

Purpose

Full length mouse ADAP1 for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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824 pGEX2T PTEN delta274-342

Plasmid

#10739

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Mutation

Deletion of amino acids 274-342.

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pCEP4-myc-HLA-A24-VN

Plasmid

#135508

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*24:02

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*24:02 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-XPLN (delta)N (aa1-117 deleted)

Plasmid

#73382

Purpose

Expresses myc tagged XPLN in mammalian cells with deletion of N-terminal amino acids 1-117

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Deleted N-terminal 1-117 aa from XPLN protein

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa304-466 (PH)

Plasmid

#73380

Purpose

Expresses amino acids 304-466 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 304-466 of XPLN (arhgef3) p…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa1-125 (N125)

Plasmid

#73378

Purpose

Expression of the N-terminal region of XPLN (amino acids 1–125, designated N125)

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses N terminal amino acid 1-125 of XPLN (ar…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
J23112-mRFP1-331Bb

Plasmid

#78278

Purpose

mRFP1 behind constitutive promoter J23112 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23112-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23103-mRFP1-331Bb

Plasmid

#78273

Purpose

mRFP1 behind constitutive promoter J23103 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23103-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23105-mRFP1-331Bb

Plasmid

#78275

Purpose

mRFP1 behind constitutive promoter J23105 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23105-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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His-MBP-NRBF2 (1-159)

Plasmid

#99331

Purpose

Mammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complex

Depositor

James Hurley

Article

Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

Insert

Nuclear receptor-binding factor 2 (NRBF2 Human)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

AA1-159 are present

Promoter

T7

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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J23101-mRFP1-331Bb

Plasmid

#78272

Purpose

mRFP1 behind constitutive promoter J23101 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23101-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pMGF157

Plasmid

#96972

Purpose

ish1-mCherry insertion cassette with Hygro resistance gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + hygro…

Promoter

N/A

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A1-VN

Plasmid

#135502

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*01:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*01:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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WT MetNI in pBAD

Plasmid

#118254

Purpose

Wild Type Methionine ABC Transporter MetNI in pBAD Vector for Transport Activity Assay

Depositor

Douglas Rees

Article

Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

Inserts

MetN (metN Escherichia coli)
MetI (metI Escherichia coli)

Tags

10x His Tag and Enterokinase Cleavage Site

Expression

Bacterial

Promoter

Arabinose Promoter and None

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-YNL

Plasmid

#65711

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, YNL

Use

Luciferase; Tol2 system

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCNL-C1_Rab11a

Plasmid

#65706

Purpose

Expresses CNL-Rab11a in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Rab11a (Rab11a Mouse)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a F656A

Plasmid

#53054

Purpose

Expresses alpha subunit of F656A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Phenylalanine 656 to Alanine

Promoter

SP6

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a Y652A

Plasmid

#53053

Purpose

Expresses alpha subunit of Y652A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Tyrosine 652 to Alanine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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