We narrowed to 13,833 results for: Cas9

1. pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti

   Plasmid

   #138318

   Purpose

   Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   SauCas9 GFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB5219

   Plasmid

   #106135

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330 EQR

   Plasmid

   #101733

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB4782

   Plasmid

   #106141

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ159

   Plasmid

   #69308

   Purpose

   Gateway entry vector with hSpCas9D10A

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   hSpCas9D10A (Human codon optimized)
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mutation

   D10A mutation; NLS fusion

   Promoter

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330A_FokI-1x7

   Plasmid

   #63607

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCF709

   Plasmid

   #121891

   Purpose

   EF1a-ZIKV-protease-mTagBFP2

   Depositor

   David Savage

   Article

   Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

   Inserts

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a core promoter

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJin-239

   Plasmid

   #125716

   Purpose

   ABA-inducible split T7 gRNA for GFP vector using RNAPN N-29-1

   Depositor

   Bryan Dickinson

   Article

   Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

   Insert

   pT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (N-29-1)-linker-ABI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PABE-2

   Plasmid

   #115623

   Purpose

   Targeted A to G in wheat plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Li et al Genome Biol. 2018 May 29;19(1):59. doi:

   Insert

   wtTadA-TadA7.10-nCas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB6581

    Plasmid

    #106137

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntF_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCfB6580

    Plasmid

    #106136

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntF_2, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCfB6575

    Plasmid

    #106133

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_2, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRS423-SgH

    Plasmid

    #163972

    Purpose

    Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQ158

    Plasmid

    #69307

    Purpose

    Gateway entry vector with hSpCas9 (Human codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    hSpCas9 (Human codon optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mutation

    NLS fusion

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330A_FokI-1x6

    Plasmid

    #63606

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHU6_Ch13_102010574-gRNA-for

    Plasmid

    #81218

    Purpose

    pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch13 reporter

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    hU6 expression of gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHU6_Ch13_102010574-gRNA-rev

    Plasmid

    #81219

    Purpose

    pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch13 reporter

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    hU6 expression of gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCF820_U6-sgRNA-EF1a-mCherry2_lenti

    Plasmid

    #138316

    Purpose

    Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SpyCas9 GFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSB1C3-hsg(M)

    Plasmid

    #138262

    Purpose

    Subcloning and expression of mCherry-targeting sgRNA M for use in Traffic Light reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    sgRNA M
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR-R4R3-mEGFP-N

    Plasmid

    #113746

    Purpose

    Gateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mEGFP
    

    Use

    CRISPR; Gateway entry vector

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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