We narrowed to 150,834 results for: ins

1. T5-sgRNA

   Plasmid

   #113655

   Purpose

   sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

   Depositor

   Jeffrey Barrick

   Article

   Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

   Insert

   promoter T5 and sgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKar2-msGFP-HDEL

   Plasmid

   #117668

   Purpose

   Localisation of Endoplasmic Reticulum

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pKar2-msGFP-HDEL
   

   Expression

   Yeast

   Mutation

   Contains the Kar2 promoter and signal sequence fr…

   Promoter

   pKar2

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-link-ymVenus-URA3

   Plasmid

   #168058

   Purpose

   Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymVenus
   

   Tags

   ymVenus

   Expression

   Yeast

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSP2283

   Plasmid

   #70702

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCOLA kiwi sensor sfGFP

   Plasmid

   #107368

   Purpose

   Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   kiwi-detecting toehold + sfGFP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. cacnb1b (rat) in pMT2 vector

   Plasmid

   #107423

   Purpose

   Expresses Calcium channel beta1b auxillary subunit in mammalian cells.

   Depositor

   Annette Dolphin

   Article

   Page et al J Neurosci. 1997 Feb 15;17(4):1330-8.

   Insert

   calcium channel beta1b auxillary subunit
   

   Expression

   Mammalian

   Promoter

   Ad MLP

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xFRB-myc

   Plasmid

   #20226

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   3xFRB (MTOR Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   1 FRB copy has a T2098L point mutation

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pCIS-krPAM2

   Plasmid

   #25457

   Depositor

   Richard Mains

   Article

   Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

   Insert

   PAM2 (Pam Rat)
   

   Expression

   Mammalian

   Mutation

   Exon A deleted (exon A extends from nt 1475 to n…

   Available Since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pPI452

   Plasmid

   #113203

   Purpose

   Inducible expression vector (dox-on) N-terminal mScarlet tagging

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Type

   Empty backbone

   Use

   Dictyostelium discoideum inducible expression vec…

   Tags

   mScarlet

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-RaACR_687-EYFP

    Plasmid

    #111077

    Purpose

    Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RaACR_687
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMBA323

    Plasmid

    #214751

    Purpose

    To create delta(glmS,nadE) working strains and maintaining their viability

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-agrBD-I

    Plasmid

    #53439

    Purpose

    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrBD locus
    

    Use

    Synthetic Biology

    Tags

    V5 epitope, 6x HIS (not expressed on agrB or agrD…

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7-lac

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. NpT7-5-ERK1

    Plasmid

    #39229

    Depositor

    Melanie Cobb

    Article

    Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

    Insert

    ERK1 (MAPK3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA-NBid-PhoCl1-CBid

    Plasmid

    #164050

    Purpose

    Optogenetic manipulation of cell apoptosis using PhoCl1

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NBid-PhoCl1-CBid
    

    Tags

    kozak sequence

    Expression

    Mammalian

    Promoter

    CMV promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. MAGE-A3Pro pGL4basic

    Plasmid

    #78138

    Purpose

    luciferase reporter plasmid

    Depositor

    Heidi Schwarzenbach

    Article

    Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

    Insert

    MAGE-A3 (MAGEA3 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    contains -418/+12 of promoter

    Promoter

    MAGE-A3

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUAS-hActin-luc2

    Plasmid

    #48081

    Purpose

    Luciferase Reporter with Gal4 DNA binding site upstream of hACTB promoter

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Luciferase
    

    Expression

    Mammalian

    Mutation

    removed hsp70 promoter, inserted amplified 615 bp…

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pDM1513

    Plasmid

    #108998

    Purpose

    safe haven knock-in plasmid for the act5 locus, N-terminal GFP tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Safe haven knock-in plasmid for the act5 locus, n…

    Tags

    GFP

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. ACS2 Truncated

    Plasmid

    #13748

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    Truncated Acetyl CoA Sythetase 2 (Acss1 Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 39–682

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pDOC-K-glmS-mPlum

    Plasmid

    #158062

    Purpose

    Donor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    monomeric plum fluoresecent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    acpP

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB80-iLID-mCherry-RAB5

    Plasmid

    #174624

    Purpose

    Optogenetic coupling to early endosomes (RAB5) via iLID

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    iLID-mCherry-RAB5 (RAB5A Synthetic, Human)
    

    Tags

    iLID-mCherry

    Expression

    Mammalian

    Mutation

    mCherry: Met1Del; RAB5: Met1Del

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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