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CD59-CD4d3+4-bio

Plasmid

#73117

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CD59 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CD59 glycoprotein (CD59, MIC11 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pBScMXT + Slo1

Plasmid

#16194

Depositor

Larry Salkoff

Article

McCobb et al Am J Physiol. 1995 Sep . 269(3 Pt 2):H76

Insert

calcium-activated potassium channel (KCNMA1, Kcnma1 Human, Mouse)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)

Plasmid

#64314

Purpose

chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells

Depositor

Noriyuki Tsumaki

Article

Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi:

Inserts

COL11A2 promoter (COL11A2 Human)
COL11A2 enhancer (COL11A2 Human)

Use

Lentiviral

Tags

EGFP and IRES-PURO

Expression

Mammalian

Mutation

N/A

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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Calbindin scFv [L109/57]

Plasmid

#182071

Purpose

Mammalian Expression of Calbindin scFv. Derived from hybridoma L109/57.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Calbindin (Homo sapiens) (CALB1 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S650A

Plasmid

#171252

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser650Ala mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S650D

Plasmid

#171253

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser650Asp mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

Promoter

CMV

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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cftr_R (OZ518)

Plasmid

#27193

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting cftr (cftr Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
cftr_L (OZ517)

Plasmid

#27192

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting cftr (cftr Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
Anti-Calbindin [L109/43R]

Plasmid

#222146

Purpose

Mammalian Expression Plasmid of anti-Calbindin (Human). Derived from hybridoma L109/43.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Calbindin (Homo sapiens) recombinant mouse monoclonal antibody. (CALB1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_EEF1A2_GTP-EFTU-D2

Plasmid

#109919

Purpose

Protein expression and purification of EEF1A2_GTP-EFTU-D2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EEF1A2_GTP-EFTU-D2 (EEF1A2, EEF1A1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-CDK6-V181I

Plasmid

#116218

Purpose

Lentiviral expression of CDK6 V181I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Mutation

V181I

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.0-Magneto2.0-p2A-mCherry

Plasmid

#74308

Purpose

Bicistronic expression of Magneto2.0 and mCherry in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)

Tags

Flag and p2A-mCherry

Expression

Mammalian

Mutation

TRPV4: delta 760–871

Promoter

CMV

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_COL3A1_CLU

Plasmid

#205794

Purpose

Express mEGFP-tagged fusion protein, COL3A1_CLU from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

COL3A1_CLU (COL3A1, CLU Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDRM55 tet-AR(1-707)

Plasmid

#183503

Purpose

Lentiviral vector expressing a doxycycline-inducible constitutively active truncated androgen receptor (wild type AR)

Depositor

Wayne Tilley

Article

Hickey et al Nat Med. 2021 Feb;27(2):310-320. doi: 10

Insert

Androgen receptor (AR Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Constitutively active truncated AR; spans AR 1-70…

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-CDK6

Plasmid

#23688

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDK6 (CDK6 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-DIO-Magneto2.0-sNRPpA

Plasmid

#74307

Purpose

Cre-dependent expression of Magneto2.0 in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)

Use

AAV and Cre/Lox

Tags

Flag

Expression

Mammalian

Mutation

TRPV4: delta 760-871

Promoter

CMV (after Cre recombination)

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-Calbindin [L109/39R]

Plasmid

#177454

Purpose

Mammalian Expression Plasmid of anti-Calbindin (Human). Derived from hybridoma L109/39.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Calbindin (Homo sapiens) recombinant mouse monoclonal antibody (CALB1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA

Plasmid

#74302

Purpose

Expression of Magneto2.0-p2A-mCherry in Rohon-Beard sensory neurons of the zebrafish

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)

Use

Zebrafish

Tags

FLAG, cmcl2::EGFP, and p2A-mCherry

Mutation

TRPV4: delta 760-871

Promoter

Neurog1

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_AR_WT

Plasmid

#82094

Purpose

Gateway Donor vector containing AR , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AR (AR Human)

Use

Gateway Cloning

Mutation

p.81_82insQQ; deletion of AA397-482

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA

Plasmid

#74306

Purpose

Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Inserts

TRPV4 (Trpv4 Rat)
ferritin (FTL and HTL1) (FTL, FTH1 Human)

Use

AAV and Cre/Lox

Tags

FLAG and p2A

Expression

Mammalian

Promoter

CMV (after cre recombination)

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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