We narrowed to 18,474 results for: MUT

1. pQdCas9.luxR(mut)-sggfp

   Plasmid

   #236185

   Purpose

   The plasmid pQdCas9.luxR(mut)-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the GFP gene. Additionally, this plasmid contains a mutation in the luxR gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   Quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
   

   Promoter

   Quorum sensing promoter

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-mNaChBacMut-T2A-tdTomato

   Plasmid

   #60657

   Purpose

   Expresses mNaChBacMut-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   mNaChBacMut-T2A-tdTomato
   

   Expression

   Mammalian

   Mutation

   E191K

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. W45A mutant of H3K9me3 biosensor

   Plasmid

   #120808

   Purpose

   FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
   

   Expression

   Mammalian

   Mutation

   Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

   Promoter

   CMV

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI-neo-RL-ZEB1 200bmutx5

   Plasmid

   #35537

   Depositor

   Greg Goodall

   Article

   Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

   Insert

   3'UTR of ZEB1 (ZEB1 Budding Yeast, Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   5 mutations in miR200b seed regions of 3'UTR…

   Available Since

   May 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-RosaR4 KKR mutations

   Plasmid

   #37199

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

   Insert

   FokI (fused to RosaR4 ZFN)
   

   Use

   Zfn expression

   Tags

   HA, ROSA26 eZFNs R4, and SV40 NLS

   Expression

   Mammalian

   Mutation

   I538K, E490K, and H537R

   Promoter

   CMV

   Available Since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hsyn-GRAB_DA-mut

   Plasmid

   #140555

   Purpose

   Expresses the DA-insensitive control sensor GRAB_DA-mut in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   DA-insensitive sensor GRAB_DA-mut (control sensor)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-FLAG-mTET2 CD mutant

   Plasmid

   #89896

   Purpose

   Expresses mouse TET2 CD mutant in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   CD (cysteine-rich, dioxygenase domain) truncation…

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-neo-RL-ZEB2 200bmut

   Plasmid

   #35538

   Depositor

   Greg Goodall

   Article

   Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

   Insert

   3'UTR of ZEB2 gene (ZEB2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   6 Mutations in the miR-200b seed regions of the Z…

   Available Since

   June 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-mCherry-CyrCR1.mutant

   Plasmid

   #128746

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
   

   Expression

   Mammalian

   Mutation

   5 point mutations disrupting the miR-7 site

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pALOD4 non-binding mutant

    Plasmid

    #111027

    Purpose

    Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

    Depositor

    Arun Radhakrishnan

    Article

    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

    Insert

    Domain 4 of Anthrolysin O
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: S404C, C472A, G501A, T502A, T503A, L5…

    Promoter

    T7

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUASTattB-dALFANb(3mut)-mGreenLantern

    Plasmid

    #219662

    Purpose

    Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern
    

    Expression

    Insect

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. K9L mutant of H3K9me3 biosensor

    Plasmid

    #120807

    Purpose

    FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
    

    Expression

    Mammalian

    Mutation

    lysine 9 is mutated to leucine 9 on histone H3

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-RosaL6 ELD mutations

    Plasmid

    #37198

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

    Insert

    FokI (fused to Rosa L6 ZFN)
    

    Use

    Zfn expression

    Tags

    HA, ROSA26 eZFNs L6, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Q486E, I499L and N496D

    Promoter

    CMV

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbdCas9S(RBSmut)_Psyn-sgRNA500

    Plasmid

    #149576

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. hRIP3 GFP RHIM mut

    Plasmid

    #41385

    Depositor

    Francis Chan

    Article

    Cho et al Cell. 2009 Jun 12;137(6):1112-23.

    Insert

    RIP3 (RIPK3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    RHIM mutant (AAAA-459-462)

    Promoter

    CMV

    Available Since

    Dec. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pRK5-mEGFP-PHOX2B-MUT

    Plasmid

    #194577

    Purpose

    Mammalian Expression of mEGFP-PHOX2B Mutant (S207Afs*102) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    PHOX2B (PHOX2B Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    S207Afs*102

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG223 PRKD1 3'UTR mut

    Plasmid

    #12042

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    PRKD1 3'UTR mut (PRKD1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pAG225 HIPK1 3'UTR mut

    Plasmid

    #12044

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    HIPK1 3'UTR mut (HIPK1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pAG177 HIPK1 3'UTR mut

    Plasmid

    #12035

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N10 3'UTR mut (HIPK1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_3xFlagEzh2 RNA binding mutant

    Plasmid

    #173718

    Purpose

    RNA-binding mutant EZH2 expression plasmid with 3XFLAG tag

    Depositor

    Thomas Cech

    Article

    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

    Insert

    EZH2 RNA-binding mutant
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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