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Plasmid#203737PurposeOverexpression of ALKBH5-MutDepositorInsertALKBH5 (ALKBH5 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationEnzyme Inactive Mutation (H226A)PromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGE144 (MutaEco; aTC-induced)
Plasmid#189824PurposeExpresses MutaEco for generating mutations in the E. coli genome (aTC-induced)DepositorInsertEcoRNAPα-PmCDA1
UseTagsExpressionBacterialMutationPromotertetR/tetA promoterAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RosaL6 ELD mutations
Plasmid#37198DepositorInsertFokI (fused to Rosa L6 ZFN)
UseZfn expressionTagsHA, ROSA26 eZFNs L6, and SV40 NLSExpressionMammalianMutationQ486E, I499L and N496DPromoterCMVAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMX-HA-UbvG08_MUT-IRES-GFP
Plasmid#75031PurposeRetroviral vector with ubiquitin variant with reverted mutations L69P and V70L (i53-DM mutant)DepositorInsertUbiquitin
UseRetroviralTagsHA and IRES-eGFPExpressionMutationUbvG08 with I44A, P69L, and L70V mutations and no…PromoterAvailable sinceJune 30, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRK5-mEGFP-FOXC1-MUT
Plasmid#194567PurposeMammalian Expression of mEGFP-FOXC1 Mutant (Q200Rfs*109) Fusion ProteinDepositorInsertFOXC1 (FOXC1 Human)
UseTagsmEGFPExpressionMammalianMutationQ200Rfs*109PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
hRIP3 GFP RHIM mut
Plasmid#41385DepositorInsertRIP3 (RIPK3 Human)
UseTagsGFPExpressionMammalianMutationRHIM mutant (AAAA-459-462)PromoterCMVAvailable sinceDec. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc mut MBS1-4
Plasmid#16565DepositorInsertc-MYC binding sites (MYC Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant MBS1-4PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGL3-OF (mutant TCF-4 sites)
Plasmid#16559DepositorInsertTCF-4 binding sites (TCF4 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutation3 copies of mutant Tcf-4 binding sitesPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
p CIneo-RL-Let7-3xBulgeB-mut
Plasmid#115369PurposeExpression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet1 CD Mut
Plasmid#72218PurposeExpress flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.DepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET1 catalytic domain (cata…PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNXF-1-5xmut_O
Plasmid#147116PurposeMammalian Expression of HsNxf-1-5xmutDepositorInsertHsNxf-1-5xmut (NXF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet3 CD Mut
Plasmid#72222PurposeExpress flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.DepositorInsertTen-Eleven Translocation 3
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET3 catalytic domain (cata…PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UBA/UIM-mut
Plasmid#187152Purposemammalian cell expression of FLAG-HUWE1 M1328A/F1330A/A1376G/S1382ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationM1328A/F1330A/A1376G/S1382APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_CRFmut
Plasmid#208663PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent mannerDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-erHRP(N175S mutant)
Plasmid#79909PurposeerHRP(N175S mutant) pCMV backboneDepositorInserterHRP(N175S mutant)
UseTagsHA tagExpressionMammalianMutationN175S mutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Marathon-RT tetramutant for Split-PE (pEH101)
Plasmid#190109PurposeExpresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)DepositorInsertMarathon-RT
UseTagsbpNLSExpressionMammalianMutationD14R, D74R, N116K, N197RPromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CRY2Clust-FUSN-mut
Plasmid#200970Purposeinduction of phase-separated condensate mediated by FUSN-IDR mutantDepositorInsertFUSNmut
UseLentiviralTagsExpressionBacterial and MammalianMutation15 Tyr-to-Ser substitutionsPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
mRIP3 GFP RHIM mut
Plasmid#41384DepositorInsertRIP3 (Ripk3 Mouse)
UseTagsGFPExpressionMammalianMutationRHIM mutantPromoterCMVAvailable sinceDec. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_OTmut
Plasmid#185389PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent mannerDepositorInsertGPCR activation based oxytocin control sensor GRAB_OTmut
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
CLXSN 5myc-CDC6-mut
Plasmid#109333Purposeexpress tagged Cdc6 mutantDepositorInsertCDC6 (CDC6 Human)
UseRetroviralTags5x MycExpressionMammalianMutationR56A, L59A, K81A, E82A, N83APromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only