We narrowed to 1,238 results for: e.coli
-
Plasmid#123291PurposeExpression of truncated Glo3 (373-459) in E.ColiDepositorAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pWDY1226
Plasmid#138593PurposeExpresses NatL2-R156A in E.coliDepositorInsertphenylacetate-coenzyme A ligase
Tags6-HisExpressionBacterialMutationchanged Arginine 156 to AlaninePromoterT7Available SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWDY1222
Plasmid#138592PurposeExpresses NatL2-K418A in E.coliDepositorInsertphenylacetate-coenzyme A ligase
Tags6-HisExpressionBacterialMutationchanged Lysine 418 to AlaninePromoterT7Available SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29b-EPN01(∆M)-His
Plasmid#84510PurposeE.coli version of EPN01(∆M)DepositorInsertEPN01(∆M)-His
TagsHis tagExpressionBacterialAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
ARK2a
Plasmid#81017PurposeE.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco
PMD
PromoterLacUV5 and Trc promoterAvailable SinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJB051 (mEos2-ZapA)
Plasmid#72650PurposeInducible expression of mEos2-ZapA in bacteriaDepositorInsertmEos2-ZapA
TagsmEos2ExpressionBacterialPromoterT5-lacAvailable SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
SNV-lacZ
Plasmid#53125Purposeretroviral vector expressing lacZ, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorInsertlacZ (lacZ E.coli)
UseRetroviral; Expression in chicken cellsPromoterSNV (spleen necrosis virus)Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1070
Plasmid#53002PurposepCon:DsrA, pLlacO-1:sodB::gfpDepositorInsertsDsrA sRNA
sodB
UseSynthetic BiologyTagsGFPExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon and pLlacO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1757
Plasmid#53037PurposePconNoHindM2:glgC::gfp, PconNoHind:RBS(st3):tetRDepositorInsertsGFP
tetR
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialPromoterPconNoHindM2 and pConNoHindAvailable SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Ile170Thr
Plasmid#50725Purposeoverexpression of the human TPI1 Ile170Thr allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
Tags6xHIS tagExpressionBacterialMutationchanged Isoleucine 170 to ThreoninePromoterT7Available SinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-hAos1
Plasmid#53135PurposeE.coli expression of full length human Aos1 (SAE1) with Nterminal His-tagDepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUB11
Plasmid#169699PurposeTemplate plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
Tags3xFLAG tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT22-GluCNZ-6His
Plasmid#213504PurposeDesigned for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.DepositorInsertGlucanase (glucan endo-1,3-beta-glucosidase)
Tags6His tagExpressionBacterialMutation1-36 Signal Sequence DeletionPromoterT7Available SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF1
Plasmid#228775PurposeProtein expression plasmid for YTH domain of YTHDF1 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDC2
Plasmid#228779PurposeProtein expression plasmid for YTH domain of YTHDC2 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF3
Plasmid#228777PurposeProtein expression plasmid for YTH domain of YTHDF3 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDC1
Plasmid#228778PurposeProtein expression plasmid for YTH domain of YTHDC1 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4 R105A
Plasmid#196072PurposeExpress mutant (R105A) SG4 in E.coliDepositorInsertSG4 R105A
Tags3XFLAG and 6XHisExpressionBacterialMutationR105A mutation in SG4PromoterT7Available SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pST-2K
Plasmid#44564Purposefor ectopic constitutive expression of two mycobacterial proteins in one vectorDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSEVA-gRic6T
Plasmid#106401PurposeE.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.DepositorInsertsgRNA
ExpressionBacterialPromoterpj23119Available SinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only