We narrowed to 16,162 results for: grna

1. pShHELIX_sgRNA_entry (CJT32)

   Plasmid

   #181782

   Purpose

   Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYLsgRNA-AtU6-29

   Plasmid

   #66203

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AV15_pCAG.Cas9.gRNA.S1

   Plasmid

   #199259

   Purpose

   Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9 nuclease
   AAVS1-targeting gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMGS7 (AAVS1sgRNA)

   Plasmid

   #126582

   Purpose

   A CRISPR/Cas9 construct to target the AAVS1 locus in humans

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   AAVS1 sgRNA
   

   Expression

   Mammalian

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLH-spsgRNA2

   Plasmid

   #64114

   Purpose

   Vector for expression of Sp sgRNA2 in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Sp sgRNA2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LsgRNA-MS2

   Plasmid

   #235597

   Purpose

   sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.

   Depositor

   Ting Zhou

   Article

   Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYLsgRNA-AtU6-1

   Plasmid

   #66202

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. U6.3>Control.gRNA.f+e

   Plasmid

   #99140

   Purpose

   Control gRNA under the regulation of an optimized chicken specific U6 promoter

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

   Insert

   Control gRNA
   

   Use

   CRISPR

   Promoter

   Chicken U6.3

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYLsgRNA-AtU3b

   Plasmid

   #66198

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   AtU3b

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. sgRNA 2 GAPDH

    Plasmid

    #83809

    Purpose

    Express Sg-2 sgRNA targeting GAPDH

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-Cj-sgRNA

    Plasmid

    #89753

    Purpose

    U6 promoter driven expression of Cj-SgRNA cloned with BsmbI

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    Cj-SgRNA
    

    Use

    Sgrna expression under u6 promoter

    Promoter

    U6

    Available Since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. sgRNA 1 GAPDH

    Plasmid

    #83808

    Purpose

    Express Sg-1 sgRNA targeting GAPDH

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKS diaCas9_sgRNA

    Plasmid

    #74923

    Purpose

    Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter

    Depositor

    Per Winge

    Article

    Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10

    Inserts

    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    

    Use

    CRISPR

    Promoter

    Cas9 module and sgRNA module

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLsCas13a-gRNA-NT

    Plasmid

    #164857

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-NT
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLH-nmsgRNA1.1

    Plasmid

    #64115

    Purpose

    Vector for expression of Nm sgRNA1.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Nm sgRNA1.1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGGDestTol2LC-4sgRNA

    Plasmid

    #64242

    Purpose

    destination vector for four U6x:sgRNA cassettes

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJC.15A.sgRNA.TA

    Plasmid

    #232483

    Purpose

    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.

    Depositor

    James Collins

    Article

    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

    Inserts

    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    P3 constitutive promoter and native promoter

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYLsgRNA-AtU3d

    Plasmid

    #66200

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Promoter

    AtU3d

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGGDestTol2LC-2sgRNA

    Plasmid

    #64240

    Purpose

    destination vector for two U6x:sgRNA cassettes

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmCherry_sgRNA-ver2

    Plasmid

    #126776

    Purpose

    gRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNA

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    sgRNA (for SpCas9)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge