We narrowed to 9,089 results for: sgRNA

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pMM117

Plasmid

#127212

Purpose

BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L1L2

Plasmid

#113735

Purpose

Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR and Gateway Cloning

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_hygro-tagBFP-lox2272

Plasmid

#162078

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

Plasmid

#74010

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5xUAS
Cas9
Cre
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere

Plasmid

#85714

Purpose

Telomere-targeting Nme sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

telomere sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158990

Purpose

Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pCMV

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP gRNA (BRDN0000563266)

Plasmid

#80036

Purpose

3rd generation lentiviral gRNA plasmid targeting EGFP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pKRG3-mU6-PUb-hSpCas9

Plasmid

#162162

Purpose

Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

N/A

Expression

Insect

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMZ376

Plasmid

#74213

Purpose

Combined sgRNA/Cas9 vector with KanMX marker

Depositor

Mikel Zaratiegui

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Promoter

adh1 and rrk1

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

Plasmid

#158988

Purpose

Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-KRAB

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-sgRNA(siteT9)

Plasmid

#154832

Purpose

sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site T9 in CHO cells

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

sgRNA(siteT9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pZR064_CROPseq-sgCD4-CRISPRi

Plasmid

#180270

Purpose

Lentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

CD4 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-FRT

Plasmid

#183241

Purpose

anhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda Red

Depositor

Karin Schnetz

Article

Rangarajan et al Microbiology (Reading). 2022 Apr;168(4).

Insert

sgRNA-FRT

Expression

Bacterial

Promoter

P-tet

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pWT055e

Plasmid

#96866

Purpose

sgRNA-EMX1

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-EMX1

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pKRG3-mU6-PUb-3xFLAG-hSpCas9

Plasmid

#162161

Purpose

Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

3xFLAG

Expression

Insect

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

Plasmid

#195504

Purpose

Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG, Puro, and T2A

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-sgCtrl_EF1a-Puro-T2A-BFP

Plasmid

#195500

Purpose

Lentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequence

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

BFP, Puro, and T2A

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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BPK2101

Plasmid

#65770

Purpose

Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized SaCas9, and SaCas9 gRNA

Use

CRISPR

Tags

NLS-3xFlag

Expression

Bacterial

Promoter

T7 (x2)

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

Plasmid

#184294

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

Depositor

Bernardo Sabatini

Insert

sgRNA-Adrb1

Use

AAV and CRISPR

Expression

Mammalian

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189922

Purpose

AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e

Use

AAV

Mutation

SaCas9 D10A

Promoter

EFS

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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