166,365 results

1. prDNA spacer-LacOx120-20xMS2-Hyg

   Plasmid

   #210220

   Purpose

   Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   120xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PiggyBac dCas9-KRAB

   Plasmid

   #191314

   Purpose

   piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Inserts

   KRAB-dCas9-HA-tagBFP
   rtTA3-IRES-PURO
   

   Use

   CRISPR

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEFIRES-P-ACSL3-mCherry

   Plasmid

   #87158

   Purpose

   Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ACSL3 (ACSL3 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1-alpha

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCXLE-SOX2-17-p2a-KLF4

   Plasmid

   #193290

   Purpose

   episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   SOX2-17-p2a-KLF4 (SOX2, KLF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SOX2-17 is engineered highly cooperative chimeric…

   Promoter

   CAG

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1 pegRNA1 with trimmed attP

   Plasmid

   #222346

   Purpose

   pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   AAVS1 attP-installation pegRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. T7opt in pCAGGS

   Plasmid

   #65974

   Purpose

   Human codon-optimized T7 polymerase

   Depositor

   Benhur Lee

   Article

   Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

   Insert

   T7-opt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15-UbE2W

   Plasmid

   #15809

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2W (UBE2W Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV Hygro DEST (w117-1)

   Plasmid

   #17454

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX313-TP53-WT

   Plasmid

   #118014

   Purpose

   Constitutive expression of wild-type human Tumor Protein p53 (TP53)

   Depositor

   David Root, William Hahn

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   TP53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lck-mScarlet-I

    Plasmid

    #98821

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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