We narrowed to 6,252 results for: nls

1. pMM0650

   Plasmid

   #129011

   Purpose

   Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
   

   Expression

   Yeast

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM0648

   Plasmid

   #129009

   Purpose

   Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1
   

   Expression

   Yeast

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM0651

   Plasmid

   #129012

   Purpose

   Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
   

   Expression

   Yeast

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. enIscB

   Plasmid

   #205410

   Purpose

   Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry
   

   Expression

   Mammalian

   Mutation

   E85R+H369R+S387R+S457R

   Promoter

   CAG, hU6, CMV

   Available Since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCH45 (multiAsCas12a-KRAB lenti)

   Plasmid

   #217330

   Purpose

   Lentiviral expression of multiAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   UCOE-SFFV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Td-CGBE

   Plasmid

   #196599

   Purpose

   Mammalian expression of Td-CGBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
   

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   TadA-8e (N46L); S. pyogenes Cas9 (D10A)

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCH4 (denAsCas12a-KRAB lenti)

   Plasmid

   #217331

   Purpose

   Lentiviral expression of denAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   denAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Mutation

   D908A/E174R/S542R/K548R

   Promoter

   UCOE-SFFV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Target-AIDmax

   Plasmid

   #139107

   Purpose

   Mammalian Target-AIDmax expression vector (pSI-626)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AXBE

   Plasmid

   #204605

   Purpose

   Mammalian expression of adenine transversion base editor AXBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   S. pyogenes Cas9 (D10A)

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGC(2.1)-U6.3

    Plasmid

    #170516

    Purpose

    PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Insert

    gRNAcore(2.1)-pU6.3
    

    Use

    Pcr template vector for amplifying grnacore(2.1)-…

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. HEpl017

    Plasmid

    #179992

    Purpose

    pCBh_NLS_TadA8e_dSiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized dSiCas12i-ABE8e driven by CBh promoter and tagmCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    TadA8e-dSiCas12i(D1049A)
    

    Expression

    Mammalian

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-dCpf1-BE0

    Plasmid

    #107678

    Purpose

    Expresses dLbCpf1-BE0 in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE0
    

    Expression

    Mammalian

    Mutation

    D832A/E925/D1148A

    Promoter

    CMV

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPK-351

    Plasmid

    #157921

    Purpose

    pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD

    Depositor

    Phillip Kyriakakis

    Article

    Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

    Inserts

    PIF3-MTAD
    IRES
    PhyB(1-621)-SV40NLS-Gal4DBD
    

    Use

    Synthetic Biology; Optogenetics

    Tags

    HA tag and SV40NLS

    Expression

    Mammalian

    Mutation

    pif3 1-523

    Promoter

    CMV

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. TadA-8e-eIscBn

    Plasmid

    #227018

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CMV promoter

    Depositor

    Dali Li

    Article

    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

    Insert

    bpNLS-TadA-8e-linker-nOgeuIscB-v3-bpNLS
    

    Mutation

    D96R/E84R/V159R/H339A

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AA197

    Plasmid

    #216049

    Purpose

    Fragmid fragment: (C' terminus) ALFA; ALFA; ALFA; ALFA; ALFA; NpNLS; 3xHA

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    ALFA_v1.3; ALFA_v1.2; ALFA_v1.1; ALFA_v1.4; ALFA_v1.5; NpNLS_v1.1; 3xHA_v1.1
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTR(EF)-tRNA(Q)

    Plasmid

    #170517

    Purpose

    PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Insert

    gRNAcore(EF)-tRNA(Q)
    

    Use

    Pcr template vector for amplifying grnacore(ef)-t…

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. THI ONLY pALD4 T-

    Plasmid

    #161497

    Purpose

    Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    

    Expression

    Yeast

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-TO-oNGN2

    Plasmid

    #198397

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

    Promoter

    CAG, EF1a, and TRE3G

    Available Since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. eiABE

    Plasmid

    #227144

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain was inserted between TadA-8e and eIscBn driven by CMV promoter

    Depositor

    Dali Li

    Article

    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

    Insert

    bpNLS-TadA-8e-linker-HMG-D-linker-nOgeuIscB-v3-bpNLS
    

    Mutation

    D96R/E84R/V159R/H339A

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. enIscB-T5E

    Plasmid

    #205411

    Purpose

    Vector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

    Insert

    pCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
    

    Expression

    Mammalian

    Mutation

    E85R+H369R+S387R+S457R

    Promoter

    CAG, hU6, CMV

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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