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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 5401 - 5420 of 6562 results

  4. B-eSpCas9

    Plasmid
    #126761
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126761-plasmid-map-sequence-id-259548

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  5. Gli3ZF in AINGpCAGGS

    Plasmid
    #121972
    Purpose
    Gli3 zinc fingers
    Depositor
    James Briscoe
    Insert
    Gli3ZF (GLI3 Human)
    Use
    Chick electroporation
    Tags
    5xmyc
    Expression
    Mammalian
    Mutation
    N terminus and C terminus deletion only nt 1411-1…
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    121972-plasmid-map-sequence-id-241924

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  6. pEMS1097

    Plasmid
    #29028
    Purpose
    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
    Depositor
    Elizabeth Simpson
    Publication
    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple201 (SLC6A5 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-Cre-NLS
    Available since
    Jan. 9, 2012
    Availability
    Academic Institutions and Nonprofits only
    29028-plasmid-map-sequence-id-13443

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  7. U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2

    Plasmid
    #214814
    Purpose
    A single vector containing a CAG-driven Cas9 variant from S. thermophilus recognizing a consensus NNACAA PAM (St1Cas9 CNRZ1066 v2) and its U6-driven sgRNA targeting human ATM
    Depositor
    Yannick Doyon
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    St1Cas9 CNRZ1066 v2 targeting ATM (ATM Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    214814-plasmid-map-sequence-id-422874

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  8. AV13_pCAG.Cas9.gRNA.NT

    Plasmid
    #199260
    Purpose
    Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9 nuclease
    Non-targeting (NT) control gRNA
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG promoter and RNA polymerase III promoter for …
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199260-plasmid-map-sequence-id-391374

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  10. B-evoSpCas9

    Plasmid
    #126765
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-evoSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    M495V; Y515N; K526E; R661Q; amino acids 1005-1013…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126765-plasmid-map-sequence-id-410921

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  11. pYPQ239-RVR

    Plasmid
    #108863
    Purpose
    FnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutations
    Depositor
    Yiping Qi
    Publication
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    FnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N623R, K629R and N633R, Cpf1 is rice codon optimi…
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108863-plasmid-map-sequence-id-274167

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  12. pTE4567

    Plasmid
    #107557
    Purpose
    Expresses human codon optimized inactive MbCpf1(dead2) in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hMbCpf1(dead2)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    E1080A
    Promoter
    CMV
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107557-plasmid-map-sequence-id-207247

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  13. nLbCas12a

    Plasmid
    #213745
    Purpose
    For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nLbCas12a
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    D156R, R1138A
    Promoter
    CMV
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213745-plasmid-map-sequence-id-422202

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  14. pDN41

    Plasmid
    #61344
    Purpose
    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)
    Depositor
    Barbara Di Ventura, Roland Eils
    Publication
    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Tags
    GS linker-AsLov2-biNLS9 and PKIt NES
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    61344-plasmid-map-sequence-id-102122

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  16. LLP780_SpyCatcher-KRAB-CO

    Plasmid
    #211773
    Purpose
    SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection
    Depositor
    Ryan Lister
    Publication
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpyCatcher-KRAB
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect KRAB e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211773-plasmid-map-sequence-id-417365

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  17. LLP784_SnoopCatcher-DNMT3A-CO

    Plasmid
    #211777
    Purpose
    SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection
    Depositor
    Ryan Lister
    Publication
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spy-EZH2
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect EZH2 e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211777-plasmid-map-sequence-id-417364

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  18. LLP789_αGCN4-DNMT3A-CO

    Plasmid
    #211783
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection
    Depositor
    Ryan Lister
    Publication
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    aGCN4-DNMT3A
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon optimised (CO) to detect DNMT3A…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    211783-plasmid-map-sequence-id-417155

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Showing: 5401 - 5420 of 6562 results