We narrowed to 5,725 results for: chia

1. pLV-dCas9-KRAB-PGK-HygR

   Plasmid

   #83890

   Purpose

   Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   humanized dead Cas9 KRAB
   aminoglycoside phosphotransferase from E. coli
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Human Ubiquitin C Promoter and mouse phosphoglyce…

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-GST-BcPC-PLC

   Plasmid

   #224255

   Purpose

   Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli

   Depositor

   Chiaki Murakami

   Article

   Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:

   Insert

   Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
   

   Use

   Tags

   GST and HRV-3C (LEVLFQGP) site

   Expression

   Bacterial

   Mutation

   The gene of Bacillus cereus ATCC 14579 was used f…

   Promoter

   lac promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pST_140_LVL2 cam

   Plasmid

   #179334

   Purpose

   NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CGBE1-NG (pBM1246)

   Plasmid

   #140256

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)_NG-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCK306

   Plasmid

   #110544

   Purpose

   rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

   Inserts

   PrhaBAD
   yfp
   rhaS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A, PrhaBAD, and kanR promoter (upstream of kanR…

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV2_CAG_oROS-HT_WPRE

   Plasmid

   #216414

   Purpose

   Encodes the genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CGBE1 (pRZ3885)

   Plasmid

   #140252

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNTI194

   Plasmid

   #84266

   Purpose

   Expresses Avi-tagged RPL1 and humanized birA

   Depositor

   Nicholas Ingolia

   Article

   Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

   Inserts

   RPL1 (RPL10A Human, (*see below))
   humanized birA
   

   Use

   Tags

   Avi tag, Flag, Gly/Ser linker, and TEV site

   Expression

   Mammalian

   Mutation

   R33A, A34E (*see below)

   Promoter

   CMV and P2A peptide

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pC1-dmito-oROS-HT

   Plasmid

   #216418

   Purpose

   Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   dmito-oROS-HT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pet16b-Avi-ubiquitin_rbs_BirA

    Plasmid

    #134897

    Purpose

    Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Inserts

    Ubiquitin (UBB Human)
    BirA
    

    Use

    Tags

    10HIS-AviTag-PreScission(3C)protease

    Expression

    Bacterial

    Mutation

    Promoter

    RBS and T7

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAcBac1-EcTrp-TGA

    Plasmid

    #228780

    Purpose

    aaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codon

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTrpRS
    EcWtR
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TGA suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pC1-oROS-HT-CaaX

    Plasmid

    #216417

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT-CaaX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDisplay-oROS-HT

    Plasmid

    #216416

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the extracellular site of cell membranes.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    pDisplay-oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pH6-TEV-spTorA-GFP(C)

    Plasmid

    #169041

    Purpose

    GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    H6-spTorA-GFP
    

    Use

    Tags

    6xHis, TEV cleavage site, and spTorA (signal pept…

    Expression

    Bacterial

    Mutation

    The mut3 GFP variant with 5 additional mutations …

    Promoter

    araBAD

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

    Plasmid

    #122850

    Purpose

    Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    m(KS_MAT)_mouseL_ACP (Fasn Mouse)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PL-5LTR-GW-A

    Plasmid

    #138480

    Purpose

    For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SECURE miniABEmax-V82G (pJUL1828)

    Plasmid

    #131313

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pELPS-eDHFR-YFP-T2A-Renilla

    Plasmid

    #193253

    Purpose

    Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase

    Depositor

    Mark Sellmyer

    Article

    Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1

    Insert

    The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    

    Use

    Lentiviral

    Tags

    Renilla luciferase (rLuc) and yellow fluorescent …

    Expression

    Mutation

    None

    Promoter

    EF1a

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

    Plasmid

    #52890

    Purpose

    "CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.

    Depositor

    Iswar Hariharan

    Article

    Bosch et al Development. 2015 Feb 1;142(3):597-606.

    Inserts

    LexGAD
    Gal4 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SECURE miniABEmax-K20A/R21A (pJUL1774)

    Plasmid

    #131312

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge