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AiP1653 - pAAV-AiE2333m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214506

Purpose

AiE2333m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1427 - pAAV-AiE2135m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214497

Purpose

AiE2135m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-Sec61β

Plasmid

#186574

Purpose

Expression of FRB-mCherry-tagged ER protein Sec61beta in mammalian cells

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

SEC61B (SEC61B Human)

Tags

FRB-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB3045(gRNA XI-3)

Plasmid

#73287

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLDPuro-MmnSR100N

Plasmid

#35171

Depositor

Benjamin Blencowe

Article

Raj et al Mol Cell. 2011 Sep 2;43(5):843-50.

Insert

Srrm4 (Srrm4 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pB-actin 16 Early

Plasmid

#13711

Depositor

Karl Munger

Article

Münger et al J Virol. 1989 Oct . 63(10):4417-21.

Insert

HPV 16 early region (E4 Human)

Expression

Mammalian

Mutation

Incl. small part of L2

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pET28-SUMO-KaiA

Plasmid

#68049

Purpose

Strain: AM5115, Used for protein purification

Depositor

Susan Golden

Article

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiA

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Mouse ZP1 (JD#264)

Plasmid

#14644

Depositor

Jurrien Dean

Article

Epifano et al Development. 1995 Jul . 121(7):1947-56.

Insert

ZP1 (Zp1 Mouse)

Expression

Bacterial

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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EXT1-pLJC2-3XFLAG

Plasmid

#215818

Purpose

Ectopic expression of EXT-1 in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-Lifeact-miRFP703

Plasmid

#178530

Purpose

Expresses Lifeact-miRFP703 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Lifeact-miRFP703

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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Ai133 (TITL-ssAPEX2tm) targeting vector

Plasmid

#114432

Purpose

Target a Cre-dependent electron microscopy tag ssAPEX2tm cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

ssAPEX2tm

Use

Cre/Lox and Mouse Targeting

Promoter

TREtight

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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dsRedm-Rab21

Plasmid

#83425

Purpose

Full-length mouse Rab21 (wildtype)

Depositor

Johanna Ivaska

Article

Pellinen et al Dev Cell. 2008 Sep;15(3):371-85. doi: 10

Insert

Rab21 (Rab21 Mouse)

Tags

dsRed-monomer

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pPA-RL-FL

Plasmid

#78863

Purpose

DULIP Tandem Construct used for data normalization

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

Firefly

Use

Expression vector

Tags

Protein A, Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pRZ-mNanog-YNL

Plasmid

#65717

Purpose

mNanog reporter plasmid (expresses YNL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

murine Nanog promoter and YNL

Use

Lentiviral and Luciferase

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM1

Plasmid

#58415

Purpose

Expresses murine IFITM1 with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-V5-Lin28A

Plasmid

#51387

Depositor

Narry Kim

Article

Heo et al Cell. 2009 Aug 21;138(4):696-708. doi: 1

Insert

Lin28A (LIN28A Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pHCMV_JEV_prME

Plasmid

#207283

Purpose

Mammalian expression of Japanese encephalitis virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

JEV_PrME (POLY Japanese encephalitis virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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PRS415-Gal1pr-sctetR-FokI

Plasmid

#44756

Depositor

Narendra Maheshri

Article

Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

Insert

sctetR-FokI (fokI Synthetic)

Expression

Yeast

Promoter

Gal1pr

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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FSW-ALDH2-T2A-EGFP

Plasmid

#197036

Purpose

Express ALDH2 from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Article

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2 (ALDH2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_MITF_P2A_Hygro_Barcode

Plasmid

#120460

Purpose

Barcoded lentiviral vector to express MITF in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MITF (MITF Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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