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ND4.2-Right DdCBE-G1397-C- T1413I

Plasmid

#179685

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Publication

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_SOD2 3'UTR

Expression

Mammalian

Mutation

T1413I

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAW-Nb127D01-mCherry

Plasmid

#171573

Purpose

Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Publication

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-mCherry (CXCR2 Human)

Tags

mCherry

Expression

Insect

Promoter

fly actin5C promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-N-EP300

Plasmid

#166452

Purpose

Expresses fusion of mCherry and EP300-N-IDR

Depositor

Shuguo Sun

Publication

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry, N-EP300(1-341) (EP300 Human)

Expression

Mammalian

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTet-O-E2F7-T2A-PuroR

Plasmid

#162348

Purpose

Lentiviral expression of E2F7 under the control of the TetON promoter

Depositor

Charles Gersbach

Publication

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

E2F7 (E2F7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAG156 Mito-FAST

Plasmid

#130723

Purpose

Expresses FAST (also called YFAST) fused to a mitochondria targeting sequence (mito) in mammalian cells

Depositor

Arnaud Gautier

Publication

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

Mito-FAST

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAM4951 (pCV0063)

Plasmid

#120084

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).

Depositor

Susan Golden

Publication

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200)

Plasmid

#119857

Purpose

Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) (YTHDF1 100-200 subdomain)

Depositor

Bryan Dickinson

Publication

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas13b-GGS-NYTHDF1(100-200)

Use

CRISPR

Expression

Mammalian

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200)

Plasmid

#119856

Purpose

Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200) (YTDHF2 100-200 subdomain)

Depositor

Bryan Dickinson

Publication

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas13b-GGS-NYTHDF2(100-200)

Use

CRISPR

Expression

Mammalian

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV14_NOP58

Plasmid

#73063

Purpose

expression clone for human NOP58 with C-terminal 3xFLAG-tag

Depositor

Stefan Stamm

Publication

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

NOP58 ribonucleoprotein (NOP58 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS2241

Plasmid

#111896

Purpose

ssAAV genome with Ple320 (POU4F1 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple320 (POU4F1 Human)

Use

AAV

Promoter

Ple320 (POU4F1 MiniPromoter)

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDisplay-GACh1.0

Plasmid

#106072

Purpose

express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

Depositor

Yulong Li

Publication

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GPCR activation based ACh sensor (GACh) 1.0

Expression

Mammalian

Promoter

CMV

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSR02

Plasmid

#69149

Purpose

Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

Depositor

Sander van den Heuvel

Publication

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

rps-27

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJZC41

Plasmid

#62332

Purpose

sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
PCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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mRuby-LaminB1-10

Plasmid

#55869

Purpose

Localization: Nuclear Envelope, Excitation: 558, Emission: 605

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LaminB1 (LMNB1 Human)

Tags

mRuby

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-Ezrin-N-14

Plasmid

#57374

Purpose

Localization: Focal Adhesions/Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ezrin (EZR Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mWasabi-LaminB1-10

Plasmid

#56507

Purpose

Localization: Nuclear Envelope, Excitation: 493, Emission: 509

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LaminB1 (LMNB1 Human)

Tags

mWasabi

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
mCerulean-TFR-20

Plasmid

#55391

Purpose

Localization: Transferin Receptor, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TFR (TFRC Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-RatLC3B-C-7

Plasmid

#55128

Purpose

Localization: Microtubule-Binding Protein, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LC3B

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
mApple-Talin-N-10

Plasmid

#54951

Purpose

Localization: Focal Adhesions, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Talin

Tags

mApple

Expression

Mammalian

Mutation

H2541R in Talin (last residue before linker)

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
mApple-Rab4a-7

Plasmid

#54943

Purpose

Localization: Ras GTPase, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab4a (RAB4A Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article