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Slc1a3-CreERT2 Targeting Vector

Plasmid

#129409

Purpose

Gene targeting vector to knock-in 2A-CreERT2 into the mouse Slc1a3 locus in place of a STOP codon

Depositor

Walker Jackson

Article

Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

Insert

Slc1a3-2A-CreERT2 Targeting Vector (Slc1a3 Mouse)

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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px335 Slc1a3 nickase 2

Plasmid

#129411

Purpose

Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

Depositor

Walker Jackson

Article

Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

Insert

sgRNA targeting Slc1a3 (Slc1a3 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3HA-AMOT p130∆PPxY1(E104K/E105K)

Plasmid

#171130

Purpose

expresses AMOT(E104K/E105K) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

Angiomotin p130 E104K/E105K (AMOT Human)

Tags

2xHA

Expression

Mammalian

Mutation

changed glutamic acid residues 104 and 105 to lys…

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-FRT-TO-Nprot_GC3opt

Plasmid

#157730

Purpose

mammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nprot_GC3opt (N SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp8_GC3opt

Plasmid

#157697

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8_GC3opt (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-FRT-TO-Nsp8-Nsp7_GC3opt

Plasmid

#157700

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp8_GC3opt

Plasmid

#157698

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp8_GC3opt (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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KC1G3_HUMAN_D0

Plasmid

#79685

Purpose

This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1G3 (CSNK1G3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-S279A

Plasmid

#60705

Purpose

retroviral expression of rat Cx43 S279A mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

S279A

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-Cx43-S279/282A

Plasmid

#60712

Purpose

mammalian expression of rat Cx43 S279/282A mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Expression

Mammalian

Mutation

S279/282A

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-Cx43-S279/282D

Plasmid

#60714

Purpose

mammalian expression of rat Cx43 S279/282D mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Expression

Mammalian

Mutation

S279/282D

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pEMS1396

Plasmid

#29194

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple146 (NTSR1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1174

Plasmid

#29088

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple240 (UGT8 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1617

Plasmid

#29279

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple146 (NTSR1 Human)

Use

Pleiades promoter project [sic, pleaides plieades…

Tags

Intron-LacZ-NLS

Available Since

Oct. 28, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1712

Plasmid

#29297

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple240 (UGT8 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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FGFR2 F276C 3xFlag

Plasmid

#110064

Purpose

FGFR2 F276C expression in mammalian cells (JCOPO paper)

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

mutated Phenylalanine 276 to Cysteine (F276C)

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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FGFR2 K41E 3xFlag

Plasmid

#110105

Purpose

expresses FGFR2 with a single amino acid change in mammalian cells

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

mutated Lysine 41 to Glutamic acid (K41E)

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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CRASP-Seq CBC Backbone

Pooled Library

#232068

Purpose

The CRASP-Seq backbone is designed to accommodate the cloning of diverse gRNA libraries. It includes a cell barcode (CBC), which enhances its functionality by enabling library preparation.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al Mol Cell. 2026 Jan 8;86(1):41-59.e15. do

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only
CRASP-Seq BE Tiling v1 empty

Pooled Library

#232070

Purpose

Tiling library to conduct CRASP-Seq experiments combined with high-throughput base editing for precise and scalable investigations into protein regions that affect splicing regulation.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al Mol Cell. 2026 Jan 8;86(1):41-59.e15. do

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only
pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

Plasmid

#174515

Purpose

Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Inserts

Mm PylS
MmtRNA-Pyl-opt(UGA)

Expression

Bacterial

Mutation

ASL from the serT gene (UGA anticodon) and recoded

Promoter

GlnS and lpp

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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