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pRPR1(1xTetO)_gRNA_handle_RPR1t

Plasmid

#62966

Purpose

aTc inducible gRNA expression vector

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Expression

Yeast

Promoter

RPR1

Available Since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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pfast NPC1L1(NTD)

Plasmid

#37366

Depositor

Johann Deisenhofer

Article

Kwon et al PLoS One. 2011 Apr 15;6(4):e18722.

Insert

npc1l1 (NPC1L1 Human)

Tags

His

Expression

Insect

Mutation

aa22-284only

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pJRL2 PHO5pr VYFP (EB#1547)

Plasmid

#14840

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter (PHO5 Budding Yeast)

Tags

VYFP

Expression

Yeast

Available Since

May 25, 2007

Availability

Academic Institutions and Nonprofits only

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pJRL2 GAL1pr VYFP (EB#1571)

Plasmid

#14847

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Tags

VYFP

Expression

Yeast

Available Since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pTE5422

Plasmid

#229031

Purpose

sfGFP-Pepper reporter: PT7-tetO-B0035-sfGFP-pepper-T7 terminator, AmpR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-tetO_B0035-sfGFP-Pepper-T7term

Expression

Bacterial

Promoter

PT7-tetO

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5428

Plasmid

#229035

Purpose

Expression vector for TetR: PT7-lacO-B0035-TetR-T7 terminator, AmpR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-RBS35-TetR-T7term

Expression

Bacterial

Promoter

PT7-lacO

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5430

Plasmid

#229037

Purpose

Azurite reporter: PT7-tetO-B0035-Azurite-T7 terminator, AmpR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-tetO-B0035-Azurite-T7term

Expression

Bacterial

Promoter

PT7-tetO

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWT-C

Plasmid

#225964

Purpose

Block V Repeats-In-Toxin (RTX) protein with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWT-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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MTF1

Plasmid

#205066

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Tang et al J Biol Chem. 2009 Feb 27;284(9):5514-22.

Insert

MTF1 (MTF1 Budding Yeast)

Expression

Bacterial

Mutation

WT

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Gm-lacZ

Plasmid

#63120

Purpose

mini-Tn7 lacZ transcriptional fusion vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

May 17, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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ER-mEGFP

Plasmid

#182876

Purpose

Localization: Endoplasmic Reticulum

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

ER-mEGFP

Expression

Mammalian

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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IRES-mCherry

Plasmid

#80139

Purpose

empty retroviral vector with IRES-mCherry

Depositor

Ellen Rothenberg

Article

Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

Type

Empty backbone

Use

Retroviral

Tags

IRES-mCherry

Expression

Mammalian

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCYC1m_yeGFP

Plasmid

#64389

Purpose

encodes yeast enhanced GFP under control of minimal pCYC1 promoter

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced GFP

Expression

Yeast

Promoter

pCYC1

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pBS185 CMV-Cre

Plasmid

#11916

Purpose

Mammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoter

Depositor

Brian Sauer

Article

Sauer et al New Biol. 1990 May . 2(5):441-9.

Insert

cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pSC101-pT7-T3RNAP

Plasmid

#140872

Purpose

T7 promoter driven synthesis of T3 RNA polymerase

Depositor

Neal Devaraj

Article

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

T3 RNA polymerase

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pU6a:sgRNA#1

Plasmid

#64245

Purpose

expresses sgRNA under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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p3962 pcDNA3-IRF3-FL

Plasmid

#22860

Depositor

Peter Howley

Article

Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

Insert

IRF3 (IRF3 Human)

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pU6b:sgRNA#3

Plasmid

#64247

Purpose

expresses sgRNA under U6b promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pU6c:sgRNA#4

Plasmid

#64248

Purpose

expresses sgRNA under U6c promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6a:sgRNA#2

Plasmid

#64246

Purpose

expresses sgRNA under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6d:sgRNA#5

Plasmid

#64249

Purpose

expresses sgRNA under U6d promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pGGDestTol2LC-5sgRNA

Plasmid

#64243

Purpose

destination vector for five U6x:sgRNA cassettes

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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pUC18R6K-mini-Tn7T

Plasmid

#64958

Purpose

mini-Tn7 base vector with transcriptional terminator

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

July 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pTXB1-CfaC-Gyra

Plasmid

#122950

Purpose

Bacterial expression of C-intein

Depositor

Thomas Muir

Article

Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

Insert

CfaC

Expression

Bacterial

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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NGFR

Plasmid

#27489

Depositor

Warren Pear

Article

Izon et al Immunity. 2001 Mar . 14(3):253-64.

Type

Empty backbone

Use

Retroviral

Tags

ECMV, IRES, and NGFR

Expression

Mammalian

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pZa-tetO-csgA-CmR

Plasmid

#89579

Purpose

Express csgA curli protein

Depositor

Urartu Seker

Article

Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

Insert

csgA

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGGDestTol2LC-4sgRNA

Plasmid

#64242

Purpose

destination vector for four U6x:sgRNA cassettes

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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pRPR1_gRNA_handle_RPR1t

Plasmid

#49014

Purpose

gRNA expression empty vector (yeast)

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

pRPR1

Available Since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pGGDestTol2LC-2sgRNA

Plasmid

#64240

Purpose

destination vector for two U6x:sgRNA cassettes

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGDestTol2LC-3sgRNA

Plasmid

#64241

Purpose

destination vector for three U6x:sgRNA cassettes

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge