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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,029 results for: CAG

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Showing: 5481 - 5500 of 10029 results

  3. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R

    Plasmid
    #185060
    Purpose
    Ef1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    185060-plasmid-map-sequence-id-375730

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  4. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Joseph Schoeniger
    Publication
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182716-plasmid-map-sequence-id-361297

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  14. lenti-EGFR-L858R-T790M-dual-epegRNA

    Plasmid
    #214100
    Purpose
    Lentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters.
    Depositor
    Ting Zhou
    Publication
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Insert
    EGFR L858R epegRNA/EGFR T790M epegRNA
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214100-plasmid-map-sequence-id-429653

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  15. LLP773_pLenti-6xHRBKET-sgRNA

    Plasmid
    #211766
    Purpose
    Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection
    Depositor
    Ryan Lister
    Publication
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
    Use
    Lentiviral
    Promoter
    pCMV and U6
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211766-plasmid-map-sequence-id-417396

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  17. pDIV515

    Plasmid
    #177703
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Publication
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    tRNA-sgRNA-tRNA
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    177703-plasmid-map-sequence-id-353317

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  19. pJEP325-pAAV-FullH1TO-SaCa9sgRNAi(CREB)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113702
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Publication
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    KASH
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113702-plasmid-map-sequence-id-229517

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  20. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid
    #195741
    Purpose
    Base editing for making a pre-stop codon in mmoX using phenol inducible system
    Depositor
    Seung-Goo Lee
    Publication
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Po (phoenol-inducible promoter)
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195741-plasmid-map-sequence-id-386423

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Showing: 5481 - 5500 of 10029 results