We narrowed to 32,648 results for: Eng

1. pMSIP122: pHelper(CyCAST)_Shuffled-spacer

   Plasmid

   #200841

   Purpose

   Inducible expression of CyCAST proteins with control crRNA for CyPSP1.

   Depositor

   Feng Zhang

   Article

   Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

   Insert

   CyCAST
   

   Expression

   Bacterial

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMZ103-rbs1-m3

   Plasmid

   #204465

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127S mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Mutation

   LasR L125W-A127S mutation

   Promoter

   In an operon and PLas

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMZ103-rbs1-m4

   Plasmid

   #204466

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Mutation

   LasR L125W-A127T mutation

   Promoter

   In an operon and PLas

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMZ103-rbs1-m5

   Plasmid

   #204467

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125F-A127G-S129N mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Mutation

   LasR L125F-A127G-S129N mutation

   Promoter

   In an operon and PLas

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMZ103-rbs1-m6

   Plasmid

   #204468

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-S129N-L130V mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Mutation

   LasR L125W-S129N-L130V mutation

   Promoter

   In an operon and PLas

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMZ103-rbs1-m7

   Plasmid

   #204469

   Purpose

   This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T-L128M mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Mutation

   LasR L125W-A127T-L128M mutation

   Promoter

   In an operon and PLas

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMZ103-rbs1-m8

   Plasmid

   #204470

   Purpose

   This is a biosensor with decreased specificity to 3OC12-HSL and improved specificity to 3OHC14-HSL, containing LasR L128F-S129W mutation.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

   Inserts

   quorum sensing regulator LasR (lasR )
   eYFP
   

   Use

   Synthetic Biology

   Mutation

   LasR L128F-S129W mutation

   Promoter

   In an operon and PLas

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNDGG007

   Plasmid

   #231311

   Purpose

   Vector part for BEVA; BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
   

   Expression

   Bacterial

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNDGG001

   Plasmid

   #231307

   Purpose

   Vector part for BEVA; BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.
   

   Expression

   Bacterial

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRExT2A-NT-BTy

    Plasmid

    #232206

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRExT2A-CT-PTy

    Plasmid

    #232203

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-PAC-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRExT2A-CT-BTy

    Plasmid

    #232209

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-BSD-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRExT2A-NT-PTy

    Plasmid

    #232202

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-Cas9-FCPF

    Plasmid

    #220132

    Purpose

    Expresses Cas9-FCPF in E.coli

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    Cas9-FCPF (cas9 S. pyogenes)
    

    Tags

    His and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. dCas9-FCPF

    Plasmid

    #220131

    Purpose

    Expresses dCas9-FCPF in mammalian cells

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    dCas9-FCPF (cas9 S. pyogenes, Synthetic)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-dCas9-FCPF

    Plasmid

    #220133

    Purpose

    Expresses dCas9-FCPF in E.coli

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    dCas9-FCPF (cas9 S. pyogenes, Synthetic)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    FCPF sequence inserted on C-terminal

    Promoter

    T7

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28Man

    Plasmid

    #203399

    Purpose

    exo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymes

    Depositor

    David Vocadlo

    Article

    Deng et al Bioorg Med Chem. 2013 Aug 15;21(16):4839

    Insert

    man2A
    

    Tags

    H6

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSyn-DIO-GCaMP6f-WPRE

    Plasmid

    #216275

    Purpose

    AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6f

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Promoter

    human Synapsin 1 (hSyn)

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJH4293

    Plasmid

    #200161

    Purpose

    Ptwk-40s GCaMP6s::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 mNeptune

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    GCaMP6
    

    Tags

    mNeptune

    Expression

    Worm

    Promoter

    Ptwk-40

    Available Since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pJH4508

    Plasmid

    #200256

    Purpose

    Pflp-18 LoxP EBFP LoxP TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    TeTx
    

    Tags

    mCherry

    Expression

    Worm

    Promoter

    Pflp-18

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge