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empty_pK7fWG2_IVD_FP611

Plasmid

#228371

Purpose

Empty dual expression vector for determining subcellular localization for protein of interest

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Type

Empty backbone

Tags

Green Florescent Protien

Expression

Plant

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCMK83

Plasmid

#226793

Purpose

Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Insert

slr0214 (slr0214 Synechocystis sp. PCC 6803)

Expression

Bacterial

Promoter

Tetracycline resistance cassette promoter (consti…

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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GB4653

Plasmid

#215267

Purpose

TU for the constitutive expression of the flippase (FLP) recombinase with a nopaline synthase (Nos) promoter

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

pNos:FLP:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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GB4829

Plasmid

#215275

Purpose

TU for the constitutive expression of the N. nambi NPGA gene under the P35S promoter and the TNOS terminator

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

P35S:NPGA:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pET29_anti-SARS-CoV-2-NM1226

Plasmid

#209422

Purpose

Express anti-SARS-CoV-2-Nanobody NM1226 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-NM1226

Tags

Myc-6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-NM1230

Plasmid

#209423

Purpose

Express anti-SARS-CoV-2-Nanobody NM1230 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-NM1230

Tags

Myc-6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-Nanobody-C5

Plasmid

#209420

Purpose

Express anti-SARS-CoV-2-Nanobody C5 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-Nanobody-C5

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-Nanobody-F2

Plasmid

#209421

Purpose

Express anti-SARS-CoV-2-Nanobody F2 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-Nanobody-F2

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGEL1022

Plasmid

#225593

Purpose

The backbone vector for rice genome editing is based on HkCas12a

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL1023

Plasmid

#225594

Purpose

The backbone vector for rice genome editing is based on PrCas12a

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL1024

Plasmid

#225595

Purpose

The Dual Pol II backbone vector for rice genome editing is based on Mb3Cas12a

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL1025

Plasmid

#225596

Purpose

The STU backbone vector for rice genome editing is based on Mb3Cas12a

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL1026

Plasmid

#225597

Purpose

The STU backbone vector for rice genome editing is based on Mb3Cas12a-R (D172R)

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Sc++

Plasmid

#198545

Purpose

Expresses human codon-optimized Sc++ and blasticidin resistance: EFS promoter-Sc++-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

Sc++

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-PARP7

Plasmid

#184474

Purpose

For gateway cloning of PARP7

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP7 (TIPARP Human)

Use

Gateway cloning

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-PARP15

Plasmid

#184471

Purpose

For gateway cloning of PARP15

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP15 (PARP15 Human)

Use

Gateway cloning

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTr2GCsgSeLEU2

Plasmid

#224870

Purpose

Disruption of S. eubayanus type LEU2 genes

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTrGCsgScLEU2

Plasmid

#224869

Purpose

Disruption of S. cerevisiae type LEU2 genes

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTrGCas having the guide sequence ScLEU2 (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTr2GCsgScLEU2

Plasmid

#224868

Purpose

Disruption of S. cerevisiae type LEU2 gene

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence ScLEU2 (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598)

Plasmid

#225432

Purpose

Transcriptional unit of BeYDV Rep/RepA (C1:C2)

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

pNOS:Rep/RepA:tNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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