We narrowed to 28,294 results for: cmv promoter

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pLenti-CMV-SMCHD1-deltaKRMR-3xFLAG

Plasmid

#130676

Purpose

Expresses human SMCHD1 deltaKRMR with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 deltaKRMR (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

K1987_R1990del

Promoter

CMV

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2(delta)KL-VSV

Plasmid

#139352

Purpose

Expression of TYK2 protein missing the kinase-like (pseudokinase) domain

Depositor

Sandra Pellegrini

Article

Velazquez et al J Biol Chem. 1995 Feb 17;270(7):3327-34.

Insert

TYK2 cDNA deleted of aa594-877 with a Tyr inserted after aa593 (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-deletion of aa 594-877

Promoter

CMV

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA1-NLS(SV40) (KAC478)

Plasmid

#133795

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized AcrIIA1

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIC1-NLS(SV40) (KAC208)

Plasmid

#134340

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIC1 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIC1

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)

Plasmid

#134341

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIC3

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2(delta)TK-VSV

Plasmid

#139353

Purpose

Expression of TYK2 protein missing the tyrosine kinase (TK) domain (aa 1-895)

Depositor

Sandra Pellegrini

Article

Velazquez et al J Biol Chem. 1995 Feb 17;270(7):3327-34.

Insert

TYK2 cDNA expressing aa 1-895 (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-deletion of aa 896-1187

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast mLST8-Flag Mut1

Plasmid

#124916

Purpose

For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L mutant

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast mLST8-Flag Mut2

Plasmid

#124917

Purpose

For stable expression_ sgRNA resistant Flag tagging mLST8 W272F/W274F mutant

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast mLST8-Flag Mut1-2

Plasmid

#124918

Purpose

For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L and W272F/W274F mutant

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

Plasmid

#119229

Purpose

Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.

Depositor

Kari Alitalo

Insert

IL3SP-Vegfd-dNdC (Vegfd Mouse)

Use

AAV

Promoter

CMV

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV7.1-Flag-NLS-hSPDEF delta2

Plasmid

#100871

Purpose

Mammalian expression of human SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

truncated protein 71-246aa

Promoter

CMV

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p7142 pHAGE-P-CMVt-N-HA-GAW-ZFYV9

Plasmid

#100162

Purpose

Expresses ZFYV9

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

ZFYV9 (ZFYVE9 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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p7054 pHAGE-P-CMVt-N-HA-GAW-COPE

Plasmid

#100144

Purpose

Expresses COPE

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

COPE (COPE Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p6330 pHAGE-P-CMVt-N-HA-GAW-HERC2-F6

Plasmid

#100140

Purpose

Encodes amino acids 4421-4834 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 4421-4834

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p6329 pHAGE-P-CMVt-N-HA-GAW-HERC2-F5

Plasmid

#100139

Purpose

Encodes amino acids 3550-4500 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 3550-4500

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p6328 pHAGE-P-CMVt-N-HA-GAW-HERC2-F4

Plasmid

#100138

Purpose

Encodes amino acids 2600-3600 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 2600-3600

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p6326 pHAGE-P-CMVt-N-HA-GAW-HERC2-F2

Plasmid

#100136

Purpose

Encodes amino acids 950-1750 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 950-1750

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p6325 pHAGE-P-CMVt-N-HA-GAW-HERC2-F1

Plasmid

#100135

Purpose

Encodes amino acids 1-1000 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 1-1000

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV7.1-Flag-NLS-hSPDEF delta3

Plasmid

#100872

Purpose

Mammalian expression of human SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

truncated protein 244-335aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV7.1-Flag-NLS-hSPDEF delta1

Plasmid

#100870

Purpose

Mammalian expression of human SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

truncated protein 1-248aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV Sport6 mouse IL-23A (1236)

Plasmid

#66915

Purpose

Mammalian expression of IL-23A

Depositor

Michael Karin

Insert

IL-23A

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/S46X without 5'UTR

Plasmid

#87730

Purpose

Expresses S46X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

S46X; 5'UTR deleted (1-190 bp)

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/S44L without 5'UTR

Plasmid

#87728

Purpose

Expresses S44L spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

S44L; 5'UTR deleted (1-190 bp)

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/L195V without 5'UTR

Plasmid

#87726

Purpose

Expresses L195V spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

L195V; 5'UTR deleted (1-190 bp)

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Tag/E112K without 5'UTR

Plasmid

#87724

Purpose

Expresses E112K spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

E112K; 5'UTR deleted (1-190 bp)

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-WRAP53beta-siRNA-resistant

Plasmid

#64680

Purpose

expresses siRNA resistant WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

FLAG

Expression

Mammalian

Mutation

siRNA resistant

Promoter

CMV

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Rem2 RNAiR2 short

Plasmid

#51596

Purpose

expresses RNAiR myc-tagged rat Rem2 (short version, missing 69 AA at N terminus)

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Rat)

Tags

myc

Expression

Mammalian

Mutation

silent mutations at shRNA 1 and 2 recognition sit…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Rem2 S69A/S334A

Plasmid

#51588

Purpose

expresses RNAiR myc-tagged Rem2 non-14-3-3 interacting

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

S69A and S334A; silent mutations at shRNA 1 and 2…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLA CMV N-Flag RALB R47

Plasmid

#50991

Purpose

Expresses RALB-R47 in mammalian cells

Depositor

Anna Sablina

Article

Simicek et al Nat Cell Biol. 2013 Oct;15(10):1220-30.

Insert

RALB (RALB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K47R

Promoter

CMV

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S127A/G385V

Plasmid

#33072

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S127A, G385V

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only