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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,189 results for: LEA

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Showing: 5521 - 5540 of 12189 results

  4. A-WDR5-1

    Plasmid
    #166515
    Purpose
    scFv of a human scaffold targeting WD repeat domain 5. Antigen coverage aa 1-334 of 334.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    April 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    166515-plasmid-map-sequence-id-327811

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  5. T-TRIM33-4

    Plasmid
    #166550
    Purpose
    scFv of a human scaffold targeting E3 ubiquitin-protein ligase TRIM33. Antigen coverage aa 882-1127 of 1127
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166550-plasmid-map-sequence-id-329241

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  6. G-SMARCA4-2

    Plasmid
    #166519
    Purpose
    scFv of a human scaffold targeting SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a4 isoform B. Antigen coverage aa 1451-1580 of 1647, Bromodomain.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166519-plasmid-map-sequence-id-329058

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  7. G-BRD4-4

    Plasmid
    #166518
    Purpose
    scFv of a human scaffold targeting Bromodomain-containing protein 4 isoform long. Antigen coverage aa 44-168 of 1362, Bromodomain 1.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166518-plasmid-map-sequence-id-329057

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  8. Z-UBQLN2-7

    Plasmid
    #166563
    Purpose
    scFv of a human scaffold targeting Ubiquilin-2. Antigen coverage aa 1-624 of 624 aa, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    166563-plasmid-map-sequence-id-327818

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  9. IBMc233

    Plasmid
    #161939
    Purpose
    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Publication
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    Expression
    Bacterial
    Promoter
    PPhlF
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161939-plasmid-map-sequence-id-327549

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  13. VRER variant

    Plasmid
    #138561
    Purpose
    Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRER
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, T1337R
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138561-plasmid-map-sequence-id-274783

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  14. VRQR variant

    Plasmid
    #138562
    Purpose
    Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRQR
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, T1337R
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138562-plasmid-map-sequence-id-274786

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  15. pOCC61

    Plasmid
    #118877
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
    Tags
    mGFP, HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118877-plasmid-map-sequence-id-232346

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  16. pOCC6

    Plasmid
    #118864
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, EGFP
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118864-plasmid-map-sequence-id-232291

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Showing: 5521 - 5540 of 12189 results