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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,938 results for: emb

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Showing: 5521 - 5540 of 11938 results

  1. AAV-CAG-dDIO-lck-smMyc-4x6T

    Plasmid
    #196421
    Purpose
    Dre-dependent AAV expression of membrane-targeted Myc spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Publication
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smMyc
    Use
    AAV; Dre/rox; astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    196421-plasmid-map-sequence-id-385348

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  2. pcDNA3.1_mGFP-FGFR3-K650E

    Plasmid
    #191757
    Purpose
    Expression of N-terminally mEGFP labeled mutated FGFR3 (isoform IIIc)-K650E mutation (associated with TD2; target mutation: c.1948A>G; silent mutation added on purpose: c.1938C>T)
    Depositor
    Irene Tiemann-Boege
    Publication
    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FGFR3IIIc (FGFR3 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    c.1948A>G (p.K650E) + silent mutation c.1938C&…
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191757-plasmid-map-sequence-id-382279

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  3. pcDNA3.1_mGFP-FGFR3-K508M

    Plasmid
    #191753
    Purpose
    Expression of N-terminally mEGFP labeled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)
    Depositor
    Irene Tiemann-Boege
    Publication
    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FGFR3IIIc (FGFR3 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    c.1523A>T (p.K508M) + silent mutation c.1515A&…
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191753-plasmid-map-sequence-id-382274

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  4. pcDNA3.1_mGFP-FGFR3-G380R

    Plasmid
    #191755
    Purpose
    Expression of N-terminally mEGFP labeled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T)
    Depositor
    Irene Tiemann-Boege
    Publication
    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FGFR3IIIc (FGFR3 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    c.1138G>A (G380R) + c.1131C>T (silent mutat…
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191755-plasmid-map-sequence-id-382281

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  5. pCMV-MCS-eDHFR(69K6)-EGFP

    Plasmid
    #172101
    Purpose
    Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP
    Depositor
    Shinya Tsukiji
    Publication
    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    22aa-eDHFR(69K6)-EGFP
    Expression
    Mammalian
    Mutation
    eDHFR(69K6): Hexalysine (K6) sequence is inserted…
    Promoter
    CMV
    Available since
    July 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172101-plasmid-map-sequence-id-340529

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  7. pTS2410-Tier1-PhCMV-Myr_mTagBFP2

    Plasmid
    #169535
    Purpose
    Tier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2
    Expression
    Mammalian
    Promoter
    PhCMV
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169535-plasmid-map-sequence-id-364986

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  10. pEF NS34a-msGFP-'Q-mito

    Plasmid
    #158764
    Purpose
    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Publication
    Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NS3/4a protease and msGFP
    Expression
    Mammalian
    Mutation
    Flexible linkers between the protease and msGFP a…
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    158764-plasmid-map-sequence-id-312950

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  11. pUASTLOTattB_mCherry-CD8-vhhGFP

    Plasmid
    #163930
    Purpose
    Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer
    Depositor
    Markus Affolter
    Publication
    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife
    Insert
    Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Insect
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    163930-plasmid-map-sequence-id-323460

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  14. pDipA-TEV-His12

    Plasmid
    #137042
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137042-plasmid-map-sequence-id-275698

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  16. phNFL-E396K

    Plasmid
    #132595
    Purpose
    Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E E396K mutation
    Depositor
    Anthony Brown
    Publication
    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NEFL (NEFL Human)
    Expression
    Mammalian
    Mutation
    1186G>A; Glu396Lys
    Promoter
    CMV
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132595-plasmid-map-sequence-id-260028

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  19. pcDNA5-eYFP-FKBP-MAD1-AA

    Plasmid
    #114038
    Purpose
    Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)
    Depositor
    Geert Kops
    Publication
    Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human MAD1 (MAD1L1 Human)
    Tags
    eYFP-FKBP12
    Expression
    Mammalian
    Mutation
    MAD2 binding deficient MAD1 K541A/L543A
    Promoter
    CMV
    Available since
    Oct. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    114038-plasmid-map-sequence-id-221144

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  20. YFP Rab4DN

    Plasmid
    #99019
    Purpose
    Localization and visualization of the dominant negative form of Rab4
    Depositor
    Narasimhan Gautam
    Publication
    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab4 DN (Rab4 Fly, Synthetic)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    S22N Dominant negative
    Promoter
    CMV
    Available since
    June 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    99019-plasmid-map-sequence-id-197862

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Showing: 5521 - 5540 of 11938 results