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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 5521 - 5540 of 16276 results

  1. AiP12556 - pAAV-AiE0410m_3xC-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2556)

    Plasmid
    #208144
    Purpose
    AiE0410m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain oligodendrocytes
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2
    Use
    AAV
    Mutation
    NA
    Promoter
    Beta Globin minimal promoter
    Available since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    208144-plasmid-map-sequence-id-410963

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  2. AiP13572 - pAAV-AiE0390h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3572)

    Plasmid
    #208143
    Purpose
    AiE0390h_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2
    Use
    AAV
    Mutation
    NA
    Promoter
    Beta Globin minimal promoter
    Available since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    208143-plasmid-map-sequence-id-410589

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  3. σ1-mCherry

    Plasmid
    #186578
    Purpose
    Expression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherry
    Depositor
    Stephen Royle
    Publication
    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AP1S1 (AP1S1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    186578-plasmid-map-sequence-id-368432

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  4. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

    Plasmid
    #127177
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector
    Depositor
    Phil Sharp
    Publication
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Entry vector with transgene for gateway cloning
    Tags
    Flag- HA- Tandem Epitopes
    Promoter
    None
    Available since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    127177-plasmid-map-sequence-id-246566

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  11. 3xFLAG-dCas9/pMSCVhyg

    Plasmid
    #134323
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134323-plasmid-map-sequence-id-430622

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  16. pX330-Flag-dSpCas9

    Plasmid
    #92113
    Purpose
    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive SpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    92113-plasmid-map-sequence-id-197502

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  17. KAP3-CT

    Plasmid
    #51396
    Purpose
    expression of EGFP-tagged COOH-terminal AA 661–793 KAP3B in mammalian cells
    Depositor
    Trina Schroer
    Publication
    Brown et al Traffic. 2005 Dec;6(12):1114-24.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Kinesin-associated protein 3 (KIFAP3 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pcDNA3.1-ERBB4-V5/HIS

    Plasmid
    #201105
    Purpose
    expression of human ERBB4 receptor tyrosine kinase in mammalian cells
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human ERBB4 receptor tyrosine kinase, full length, wildtype (ERBB4 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201105-plasmid-map-sequence-id-394497

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  20. pKrox24(MapErk)dTomato

    Plasmid
    #200113
    Purpose
    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MapErk sequence in minimal promoter
    Expression
    Mammalian
    Mutation
    highly modified sequence
    Promoter
    5 copies of designed MapErk sequence, no other pr…
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    200113-plasmid-map-sequence-id-393688

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Showing: 5521 - 5540 of 16276 results