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pAS2-1-Brr2

Plasmid

#99995

Purpose

Express yeast Brr2 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Brr2 (BRR2 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pACT2-Rcl1

Plasmid

#99992

Purpose

Express yeast Rcl1 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Rcl1 (RCL1 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Mutation

Contains amino acids 1-217 of Rcl1

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Kre33

Plasmid

#99989

Purpose

Express yeast Kre33 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Kre33 (KRE33 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only
CC1-Pos6

Plasmid

#101656

Purpose

CC1-Pos6 is a modified version of CC1-M240 that has 4 arginines in its linker. (* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Article

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Insert

CC1-Pos6

Expression

Mammalian

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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CC1-M240

Plasmid

#101657

Purpose

CC1 with shorter linker length and the 'GDP' fusion site. (* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Article

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Insert

CC1-M240

Expression

Mammalian

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCML 220

Plasmid

#98588

Purpose

pCYFP. Expresses CYFP control fusion in E. coli for TriFC assay (kanR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Type

Empty backbone

Expression

Bacterial

Promoter

pLacO

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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LPhP-pHG165

Plasmid

#90047

Purpose

Constitutive expression of a chimeric LacI:PurR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Tungtur et al J Mol Biol. 2010 Jan 29;395(4):785-802.

Insert

LPhP

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a_Msm_RbpA

Plasmid

#87928

Purpose

Mycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposes

Depositor

Seth Darst

Article

Hubin et al Elife. 2017 Jan 9;6. pii: e22520. doi: 1

Insert

RbpA

Expression

Bacterial

Promoter

T7P

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP

Plasmid

#84253

Purpose

Expresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP

Plasmid

#84252

Purpose

Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-myc-mCE

Plasmid

#82470

Purpose

Expression of myc epitope and murine mRNA Capping Enzyme in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Capping Enzyme (Rngtt Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pJK_proNR2_LGK.9

Plasmid

#81162

Purpose

Expresses levoglucosan kinase variant LGK.9 with promoter proNR2

Depositor

Timothy Whitehead

Article

Klesmith et al ACS Synth Biol. 2015 Sep 14.

Insert

Levoglucosan kinase

Tags

His6x

Expression

Bacterial

Mutation

LGK.9 - (T15R, A57Q, R94G, R100C, N101G, S105R, L…

Promoter

proNR2

Available Since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Lf-MetQ

Plasmid

#78305

Purpose

Methionine Binding Protein (MetQ) with C-Terminal 6x Histidine Tag

Depositor

Douglas Rees

Article

Nguyen et al Biol Chem. 2015 Sep;396(9-10):1127-34. d

Insert

Methionine Binding Protein (metQ )

Tags

6x Histidine Tag

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pJL580

Plasmid

#78185

Purpose

Ce rpy-1 expression in worms

Depositor

Junho Lee

Article

Nam et al J Biol Chem. 2009 Mar 20;284(12):8195-20

Insert

rpy-1 (rpy-1 Nematode)

Expression

Worm

Promoter

rpy-1

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
YCp33-YDL183c-HA

Plasmid

#70164

Purpose

yeast expression of YDL183c from its own promoter and in fusion with a triple HA epitope at the C-terminal end

Depositor

Karin Nowikovsky

Article

Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41

Insert

YDL183c

Tags

3xHA

Expression

Yeast

Promoter

YDL183c (217 nucleotides upstream of the ATG)

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only
pDGB1_omega2R

Plasmid

#68237

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET11a+T214V

Plasmid

#66134

Purpose

Expresses the T214V mutant of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Expression

Bacterial

Mutation

T214V

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCVD531

Plasmid

#66160

Purpose

A 8.9 kb HindIII fragment carrying the tdh-like gene of Vibrio hollisae strain 9041 was cloned into the HindIII site of pBR322.

Depositor

James Kaper

Article

Nishibuchi et al FEMS Microbiol Lett. 1990 Feb;55(3):251-

Insert

tdh-like

Use

Cloning vector

Promoter

Unknown

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pJZC103

Plasmid

#62340

Purpose

sgRNA for mammalian cells with mCherry marker

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-20nmSAH-GFP(1-10)

Plasmid

#61661

Purpose

20nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-20nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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