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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 48,920 results for: des.1

Showing: 5541 - 5560 of 48920 results

  3. pLeGO.sgGata1.4.RUNX1A.iG2

    Plasmid
    #181976
    Purpose
    gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)
    Depositor
    Jan-Henning Klusmann
    Inserts
    GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
    RUNX1A (RUNX1 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Promoter
    Available since
    April 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181976-plasmid-map-sequence-id-358965

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  6. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid
    #111903
    Purpose
    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.
    Depositor
    Andrew Carter
    Inserts
    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    Use
    Tags
    8xHis, SNAPf-tag, TEV site, and ZZ-tag
    Expression
    Insect
    Mutation
    Optimised for expression in Sf9 cells.
    Promoter
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111903-plasmid-map-sequence-id-215307

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  7. pRRL.PPT.SFFV.HA.RUNX1C.IRES.eGFP

    Plasmid
    #181980
    Purpose
    Human RUNX1C gene overexpression
    Depositor
    Jan-Henning Klusmann
    Insert
    RUNX1C (human) (RUNX1 Human)
    Use
    Lentiviral
    Tags
    HA (Human influenza hemagglutinin)
    Expression
    Mutation
    Promoter
    Available since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    181980-plasmid-map-sequence-id-455918

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  11. pZac2.1 gfaABC1D-cyto-GCaMP6f (AAV5)

    Viral Prep
    #52925-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pZac2.1 gfaABC1D-cyto-GCaMP6f (#52925). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-cyto-GCaMP6f plasmid DNA. gfaABC1D-driven (similar to GFAP promoter) cyto-GCaMP6f calcium sensor expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Baljit Khakh
    Article
    Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1
    Promoter
    Tags
    None
    Available since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only

  12. pAAV-hSynapsin- soCoChR-GFP (AAV9)

    Viral Prep
    #107708-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSynapsin- soCoChR-GFP (#107708). In addition to the viral particles, you will also receive purified pAAV-hSynapsin- soCoChR-GFP plasmid DNA. Synapsin-driven somatic CoChR (soCoChR) fused to GFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.
    Promoter
    Syn
    Tags
    GFP
    Available since
    Sept. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. pAAV-hSynapsin-FLEX-soCoChR-GFP (AAV9)

    Viral Prep
    #107712-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSynapsin-FLEX-soCoChR-GFP (#107712). In addition to the viral particles, you will also receive purified pAAV-hSynapsin-FLEX-soCoChR-GFP plasmid DNA. Synapsin-driven, Cre-dependent somatic CoChR (soCoChR) fused to GFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.
    Promoter
    Syn
    Tags
    GFP (Cre-dependent)
    Available since
    Sept. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. pChlr-1SE

    Plasmid
    #52140
    Purpose
    Encodes module 1 with amino acids 12S-16E for recognition of nt 8G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 16Q to 16E in Module 1
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pChlr-1SR

    Plasmid
    #52142
    Purpose
    Encodes module 1 with amino acids 12S-16R for recognition of nt 8C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 16Q to 16R in Module 1
    Promoter
    Available since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pChlr-1NQ

    Plasmid
    #52141
    Purpose
    Encodes module 1 with amino acids 12N-16Q for recognition of nt 8U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12S to 12N in Module 1
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5541 - 5560 of 48920 results