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pGL2B -376/+64

Plasmid

#24945

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pET28a-TGFα-GSY-SPM

Plasmid

#163615

Purpose

Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.

Depositor

Mark Howarth

Article

Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

Insert

TGFa-GSY-SPM (TGFA Synthetic, Human)

Tags

C-tag and His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Cys-ELP(120nm)-LPETGG

Plasmid

#90472

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

Cys-ELP(120nm)-LPETGG

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-GGG-ELP(120nm)-Cys

Plasmid

#91572

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(120nm)-Cys

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDonor_ISCro4_(WT)_Donor_ECFP

Plasmid

#251501

Purpose

Donor plasmid for monitoring insertion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL and Tol2_ISCro4_Insertion_Reporter plasmid

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4_(WT)_Donor sequence and ECFP

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pDONR223_DNASE2_WT

Plasmid

#81815

Purpose

Gateway Donor vector containing DNASE2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DNASE2 (DNASE2 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-DYRK1B

Plasmid

#101771

Purpose

Mammalian expression of DYRK1B fusion protein

Depositor

Michael Huen

Article

Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

Insert

DYRK1B / MIRK (DYRK1B Human)

Tags

S peptide-flag-streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

siRNA-resistant allele

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only
Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37b, 46]

Plasmid

#26570

Depositor

Diane Lipscombe

Article

Lin et al Neuron. 1997 Jan . 18(1):153-66.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 24a. Contains …

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
AAVS1-idCas9-KRAB-Hygro donor

Plasmid

#199621

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

Insert

AAVS1-idCas9-KRAB-Hygro

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PhoDer

Plasmid

#188473

Purpose

Expresses the optogenetic human Gasdermin D (PhoDer) in mammalian cells

Depositor

Gary Mo

Article

Santa Cruz Garcia et al Nat Commun. 2022 Jan 10;13(1):52. doi: 1

Insert

PhoDer (GSDMD Synthetic, Human)

Expression

Mammalian

Promoter

CMV IE

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pRB133

Plasmid

#180176

Purpose

GluN2A in pCDNA3.1

Depositor

Richard Bergeron

Article

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN2A (Grin2a Rat)

Expression

Mammalian

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pmirGLO:Δluc::gfp ΔAmpR::KanR

Plasmid

#176640

Purpose

MPRAu backbone vector

Depositor

Pardis Sabeti

Article

Griesemer et al Cell. 2021 Sep 30;184(20):5247-5260.e19.

Insert

emGFP

Expression

Mammalian

Promoter

Human PGK-1

Available Since

Feb. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pIA1385

Plasmid

#166862

Purpose

Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM51559

Plasmid

#153226

Purpose

Control plasmid for knockout of TRY and CPC genes

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Cas9, guide RNA for TRY and CPC, FAST marker

Use

Synthetic Biology

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pSL0915 (CRISPR with target 3)

Plasmid

#130643

Purpose

Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

CRISPR(target-3)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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SLF060: Lenti-CaMKIIa-jRGECO1a

Plasmid

#112282

Purpose

For lentiviral or direct expression of cytosolic jRGECO1a in excitatory neurons

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

jRGECO1a

Use

Lentiviral

Tags

NES-6xHIS

Expression

Mammalian

Promoter

CaMKIa

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_IRF4_WT

Plasmid

#81782

Purpose

Gateway Donor vector containing IRF4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRF4 (IRF4 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERRFI1_WT

Plasmid

#82116

Purpose

Gateway Donor vector containing ERRFI1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERRFI1 (ERRFI1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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GIPR-DuET

Plasmid

#213252

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GIPR (GIPR Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-IKZF3-NTERM-GFP

Plasmid

#185764

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, IKZF3 minidegron

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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