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pETDuet1-LanCL2-H264A-C-Ter-His

Plasmid

#154187

Purpose

To express LanCL2-H264A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

His264->Ala264

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet1-LanCL2-C321A/C367A-C-Ter-His

Plasmid

#154188

Purpose

To express LanCL2-C321A/C367A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Cys321->Ala321; Cys367->Ala367

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b-LanCL1-H219A

Plasmid

#154190

Purpose

To express LanCL1-H219A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL1

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

His219->Ala219

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b-LanCL1-C322A

Plasmid

#154191

Purpose

To express LanCL1-C322A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL1

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Cys322->Ala322

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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CEA-N domain residues 1-132 (CEACAM5)-pET30b

Plasmid

#174693

Purpose

Plasmid expressing CEA-N domain

Depositor

Jean Gariepy

Article

Abdul-Wahid et al Int J Cancer. 2016 Aug 15;139(4):841-53.

Insert

CEA-N Domain

Use

Tags

TEV Cleavage Site and hexahistidine (HIS) tag

Expression

Bacterial

Mutation

none

Promoter

T7 Promoter

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPICZ-aC-MSVU-BZ

Plasmid

#160426

Purpose

Expression of c-luciferase from sp. Maristella sp. SVU (Belize)

Depositor

Todd Oakley

Article

Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

Insert

luciferase

Use

Luciferase

Tags

6x Histidine tag, Yeast alpha secretion factor, c…

Expression

Yeast

Mutation

See depositor comments below

Promoter

AOX

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X72-527: His6-tev-Hs.CALM1(76-148)

Plasmid

#159695

Purpose

E. coli protein expression of His6-tev-Hs.CALM1(76-148)

Depositor

Dominic Esposito

Article

Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

Insert

His6-tev-Hs.CALM1(76-148) (CALM1 Human)

Use

Tags

His6-tev

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/181/666E

Plasmid

#105314

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S159/181/666E

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/666E

Plasmid

#105313

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S159/666E

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP KInd2 S159E

Plasmid

#105311

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S159E

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/666A

Plasmid

#105309

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S159/666A

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S666A

Plasmid

#105308

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S666A

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP KInd2 S159A

Plasmid

#105306

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S159A

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_Cas9–superNLS

Plasmid

#232430

Purpose

Expression plasmid for the SpCas9 nuclease with superNLS

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

Cas9–superNLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_empty-sgRNA(PP7)

Plasmid

#232432

Purpose

empty gRNA backbone which contains the PP7 aptamer in the scaffold tetraloop

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

PP7-tagged sgRNA scaffold driven by human U6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-mCherry-G12V-KRAS-IRES-Blast

Plasmid

#153336

Purpose

Lentiviral vector plasmid for integration and constitutive expression of mCherry fused to oncogenic G12V-KRAS in mammalian cells

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

mCherry-G12V-KRAS (KRAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_iPE-C_P2A_Csy4

Plasmid

#232428

Purpose

Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

iPE-C–P2A–Csy4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_rd6_pegRNA_(PP7-C4-Q1)

Plasmid

#232433

Purpose

pegRNA with optimized 3' modifications to correct the rd6 mutation

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_rd6_nsgRNA(PP7)

Plasmid

#232434

Purpose

nsgRNA for PE3b correction of the rd6 mutation, contains a PP7 in the scaffold tetraloop

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

PP7-tagged nicking gRNA (PE3b) for rd6 correction driven by human U6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_ABE8e–superNLS

Plasmid

#232429

Purpose

Expression plasmid for the ABE8e base editor with superNLS

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

ABE8e–superNLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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