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Plasmid#178852PurposeMammalian expression of miRFP670-eDHFR(69K6)DepositorInsertmiRFP670-eDHFR(69K6)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-mNG-eDHFR(69K6)
Plasmid#178853PurposeMammalian expression of mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Theo-LUCID
Plasmid#119048PurposePlasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Theo-LUCID_part1
Fab_Theo-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Quin-LUCID
Plasmid#119049PurposePlasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Quin-LUCID_part1
Fab_Quin-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_MTX-LUCID
Plasmid#119047PurposePlasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_MTX-LUCID_part1
Fab_MTX-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
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DHB-mVenus
Plasmid#136461PurposeFluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2DepositorInsertDNA Helicase B (HELB Human)
UseLentiviralTagsmVenusExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-GFP
Plasmid#11154PurposeMammalian expression vector for expression of GFP (EF1a promoter)DepositorUseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo
Plasmid#139449PurposeLentiviral vector with gRNA scaffold and neomycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: neoR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1_Neo
Plasmid#125593PurposeLentiviral expression of sgRNA with GFP and neomycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT-IRES-Puro
Plasmid#182590Purposeexpresses TERT in mammalian cellsDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_PE7-2A-BSD
Plasmid#234073PurposeFor lentiviral expression of PE7 with blasticidin resistanceDepositorInsertPE7
UseCRISPR and LentiviralTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF-1α core promoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Cas9_Blast
Plasmid#125592PurposeLentiviral expression of spCas9 with blasticidin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC034 - LwCas13a-msfGFP-2A-Blast
Plasmid#91924PurposeExpresses active LwCas13a for mammalian RNA targeting with Blasticidin selection markerDepositorInsertLwCas13a
UseCRISPR and LentiviralTagsNLS and msfGFP-NLS-3xHA, P2A, BlasticidinExpressionMammalianMutationPromoterEFSAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC0056 - LwCas13a-msfGFP-NES
Plasmid#105815PurposeExpresses active LwaCas13a-NES for mammalian RNA targetingDepositorInsertLwCas13a
UseCRISPR and LentiviralTagsNES and msfGFP-NES-3xHAExpressionMammalianMutationPromoterEF-1aAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Cas9_puro
Plasmid#108100PurposeLentiviral expression plasmid of spCas9 with puromycin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEFIRES-P-mCherry-HPos
Plasmid#87159PurposeFluorescent marker of lipid droplets (LDs)DepositorInsertHPos
UseTagsmCherryExpressionMammalianMutationPromoterEF-1-alphaAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEBB Flag GCN5
Plasmid#74784Purposeexpress of GCN5 in mammalian cellsDepositorInsertGCN5 (KAT2A Human)
UseTagsFlagExpressionMammalianMutationPromoterEF-1alpha promoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRDA_816
Plasmid#201157PurposeEnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRaDepositorInsert5x-ALFA tag-dEnAsCas12a-VP64
UseCRISPR and LentiviralTagsExpressionMutationD908APromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-dCas9-TurboID-blast
Plasmid#222599PurposeExpress fussed dCas9-TurboID for proximity labelingDepositorInsertdCas9-TurboID
UseLentiviralTagsExpressionMutationPromoterEF-1αAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV-PGKpuro2ABFP
Plasmid#122372PurposeExpresses puro-2A-BFP in mammalian cellsDepositorInsertPuro-2A-BFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC035 - dLwCas13a-msfGFP
Plasmid#91925PurposeExpresses catalytically inactive LwCas13a for mammalian RNA targetingDepositorInsertdLwCas13a
UseCRISPRTagsNLS-3xHA and NLS-msfGFPExpressionMammalianMutationR488A and R1060APromoterEF-1aAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_LKB1
Plasmid#108111PurposeLentiviral expression plasmid of human LKB1 cDNA with neomycin resistance geneDepositorInsertLKB1 (STK11 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBB HA RelA
Plasmid#74892PurposeExpress RelA in mammalian cellsDepositorInsertRelA (RELA Human)
UseTagsHAExpressionMammalianMutationPromoterEF-1alpha promoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MCAM-GFP
Plasmid#214475PurposeLentiviral vector for the expression of MCAM-GFP fusion protein in mammalian cells.DepositorInsertMCAM (MCAM Human)
UseLentiviral; GatewayTagsEGFPExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV EFIa DIO LMO11 (GeNL_SS-ChRmine)
Plasmid#219426PurposeCre-dependent expression of luciferase opsin fusion protein for bioluminescent optogeneticsDepositorInsertmNeonGreen, ISN-IE, ChRmine
UseAAVTagsGaussia luciferase signal sequenceExpressionMutationPromoterEF-1alphaAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWPI-N1ICD
Plasmid#185525PurposeConstitutive overexpression of human NOTCH1 intracellular domainDepositorInsertNOTCH1 intracellular domain (NOTCH1 Human)
UseLentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWPI-N3ICD
Plasmid#185524PurposeConstitutive overexpression of human NOTCH3 intracellular domainDepositorInsertNOTCH3 intracellular domain (NOTCH3 Human)
UseLentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_PEmax-2A-MLH1dn-2A-BSD
Plasmid#234072PurposeFor lentiviral expression of PEmax with MLH1dn and blasticidin resistanceDepositorInsertPEmax-2A-MLH1dn
UseCRISPR and LentiviralTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF-1α core promoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDA_763
Plasmid#201156PurposeEnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' NLS for CRISPRaDepositorInsert5x-ALFA tag-dEnAsCas12a
UseCRISPR and LentiviralTagsExpressionMutationD908APromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2 NT sgRNA3
Plasmid#164929PurposeExpresses Non-targeting sgRNA3 control in mammalian cellsDepositorInsertNon-targeting sgRNA3 (verified for knockout)
UseLentiviralTagsExpressionMutationPromoterEFS promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_gRNA -hMLHdn-Puro
Plasmid#191105PurposeBackbone for lentiviral pegRNA library construction with hMLH1dn and Puro markerDepositorInserthMLH1dn
UseCRISPR and LentiviralTagsExpressionMammalianMutationI31 silent mutation (ATA to ATC) to remove early …PromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a
Plasmid#31483DepositorInsertTraffic Light Reporter 2.1 (V2468 zfn target) EF Puro
UseLentiviralTagsExpressionMutationTraffic Light 2.0 series have a +3 PEST sequence …PromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2 EGFP sgRNA2
Plasmid#164933PurposeExpresses EGFP sgRNA2 in mammalian cellsDepositorInsertsgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
UseLentiviralTagsExpressionMutationPromoterEFS promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3
Plasmid#108103PurposeLentiviral expression plasmid of human SIK3 cDNA with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flp-DOG-NW
Plasmid#75469PurposeFlp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1InsertFlp-DOG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAXEF-Rap1-E63(GTP)
Plasmid#32698DepositorInsertRAP1 (RAP1A Human)
UseTagsExpressionMammalianMutationE63: Consitutively GTP-boundPromoterEF promoterAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A
Plasmid#110555PurposepFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.DepositorInsertAnti-RD114 heavy chain
UseTagsmIgG2AExpressionBacterial and MammalianMutationPromoterEF-1 alpha core promoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2 EGFP sgRNA1
Plasmid#164932PurposeExpresses EGFP sgRNA1 in mammalian cellsDepositorInsertsgRNA1 targeting Enhanced green fluorescent protein (verified for knockout)
UseLentiviralTagsExpressionMutationPromoterEFS promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only