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Human Genome-wide Intron Tagging Library, Frame 1

Pooled Library

#216134

Purpose

Library of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only
Human Genome-wide Intron Tagging Library, Frame 0

Pooled Library

#216133

Purpose

Library of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only
Oxford Drosophila melanogaster Genome-wide CRISPR Library

Pooled Library

#64750

Purpose

A genome-wide CRISPR library covering 13,501 genes (98.8% of the Drosophila melanogaster genome), among which 8,989 genes are targeted by three or more independent single guide RNAs (sgRNAs).

Depositor

Ji-Long Liu

Article

Bassett et al J Genet Genomics. 2015 Jun 20;42(6):301-

Expression

Insect

Species

Drosophila melanogaster, Streptococcus pyogenes, and Synthetic

Use

CRISPR

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
Human Brunello kinome pooled library, guides 1-8 in lentiCRISPRv2 backbone

Pooled Library

#1000000083

Purpose

CRISPR gRNA knockout pooled library for targeting the human kinome

Depositor

John Doench, David Root

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Mouse genome-wide lentiviral CRISPR gRNA library version 2

Pooled Library

#67988

Purpose

Mouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Species

Homo sapiens, Mus musculus, and Synthetic

Use

CRISPR and Lentiviral

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only
Mouse Brie kinome pooled library, guides 1-4 in lentiCRISPRv2 backbone

Pooled Library

#75317

Purpose

Mouse sgRNA library in backbone lentiCRISPRv2 targeting 713 kinase genes and containing 2,852 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Human genome-wide lentiviral CRISPR gRNA library version 1

Pooled Library

#67989

Purpose

Human genome-wide CRISPR guide RNA library V1 (pKLV2-U6gRNA5(HumanV1)-PGKpuro2ABFP-W)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Species

Homo sapiens, Mus musculus, and Synthetic

Use

CRISPR and Lentiviral

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only
Human Brunello kinome pooled library, guides 1-4 in lentiCRISPRv2 backbone

Pooled Library

#75314

Purpose

Human sgRNA library in backbone lentiCRISPRv2 targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Human Brunello kinome pooled library, guides 5-8 in lentiCRISPRv2 backbone

Pooled Library

#75315

Purpose

Human sgRNA library in backbone lentiCRISPRv2 targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Mini-human AsCpf1-based human genome-wide knockout library

Pooled Library

#130630

Purpose

Expresses crRNAs for genome-wide knockout pooled library screening

Depositor

Giulio Draetta

Article

Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only
mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFP

Pooled Library

#197348

Purpose

To allow for genome-wide genetic modifier screening, the library has a second fixed non-targeting control guide.

Depositor

Jonathan Weissman, Rebecca Voorhees

Article

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Species

Homo sapiens

Use

Lentiviral

Available since

May 25, 2023

Availability

Academic Institutions and Nonprofits only
mU6-CR1-CRISPRiv2_hU6-CR3-EMC2-guide_EF1a-PuroR-T2A-BFP

Pooled Library

#197349

Purpose

To allow for genome-wide genetic modifier screening, the library has a second fixed guide targeting EMC2.

Depositor

Jonathan Weissman, Rebecca Voorhees

Article

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Species

Homo sapiens

Use

Lentiviral

Available since

May 25, 2023

Availability

Academic Institutions and Nonprofits only
Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in lentiCRISPR v1

Pooled Library

#1000000100

Purpose

CRISPR gRNA knockout pooled library (version 1) for targeting the human genome

Depositor

David Sabatini, Eric Lander

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
Sabatini/Lander Lab Activity-Optimized Genome-wide Mouse sgRNA Library in pLenti-sgRNA

Pooled Library

#1000000096

Purpose

CRISPR gRNA knockout pooled library for targeting the mouse genome

Depositor

David Sabatini, Eric Lander

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in pLenti-sgRNA

Pooled Library

#1000000095

Purpose

CRISPR gRNA knockout pooled library for targeting the human genome

Depositor

David Sabatini, Eric Lander

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
pRSpuro shID1#6

Plasmid

#19165

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 1 (ID1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
Wei lab paired-guide RNA (pgRNA) CRISPR–Cas9 library for human long non-coding RNAs (lncRNAs)

Pooled Library

#89640

Purpose

Paired sgRNA library containing 12,472 pgRNAs targeting 671 human long non-coding RNAs (lncRNAs)

Depositor

Wensheng Wei

Article

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Expression

Mammalian

Use

Lentiviral

Available since

May 17, 2017

Availability

Academic Institutions and Nonprofits only
pAAV-S5E2-GCaMP6f (AAV9)

Viral Prep

#135632-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Promoter

Tags

None

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only
pAAV-S5E2-GCaMP6f (AAV1)

Viral Prep

#135632-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Promoter

Tags

None

Available since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only
pAAV-S5E2-Gq-P2A-dTomato (AAV9)

Viral Prep

#135635-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-S5E2-Gq-P2A-dTomato (#135635). In addition to the viral particles, you will also receive purified pAAV-S5E2-Gq-P2A-dTomato plasmid DNA. Expression of Gq-DREADD and dTomato (physically separate) under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Promoter

Tags

dTomato (physically separate, not a fusion protein)

Available since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

Showing: 541 - 560 of 29625 results

Results per page:

Sort results by:

Human Genome-wide Intron Tagging Library, Frame 1

Human Genome-wide Intron Tagging Library, Frame 0

Oxford Drosophila melanogaster Genome-wide CRISPR Library

Human Brunello kinome pooled library, guides 1-8 in lentiCRISPRv2 backbone

Mouse genome-wide lentiviral CRISPR gRNA library version 2

Mouse Brie kinome pooled library, guides 1-4 in lentiCRISPRv2 backbone

Human genome-wide lentiviral CRISPR gRNA library version 1

Human Brunello kinome pooled library, guides 1-4 in lentiCRISPRv2 backbone

Human Brunello kinome pooled library, guides 5-8 in lentiCRISPRv2 backbone

Mini-human AsCpf1-based human genome-wide knockout library

mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFP

mU6-CR1-CRISPRiv2_hU6-CR3-EMC2-guide_EF1a-PuroR-T2A-BFP

Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in lentiCRISPR v1

Sabatini/Lander Lab Activity-Optimized Genome-wide Mouse sgRNA Library in pLenti-sgRNA

Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in pLenti-sgRNA

pRSpuro shID1#6

Wei lab paired-guide RNA (pgRNA) CRISPR–Cas9 library for human long non-coding RNAs (lncRNAs)

pAAV-S5E2-GCaMP6f (AAV9)

pAAV-S5E2-GCaMP6f (AAV1)

pAAV-S5E2-Gq-P2A-dTomato (AAV9)

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