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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,273 results for: Met;

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Showing: 541 - 560 of 19273 results

  4. pDCAF3-IS5

    Plasmid
    #65220
    Purpose
    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.
    Depositor
    Jeffrey Barrick
    Article
    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.
    Inserts
    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Changed start codon from gtg to atg and Changed s…
    Promoter
    BBa_J23100
    Available Since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    65220-plasmid-map-sequence-id-134450

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  5. pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc

    Plasmid
    #129105
    Purpose
    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production
    Depositor
    Eleftherios Terry Papoutsakis
    Article
    Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10
    Insert
    BsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
    Expression
    Bacterial
    Available Since
    Nov. 11, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129105-plasmid-map-sequence-id-255085

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  6. pCMV-dCas13-inactive M3M14nes

    Plasmid
    #157855
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    David Liu
    Article
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Insert
    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    157855-plasmid-map-sequence-id-307628

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  13. pBS NL4-3 envFS eGFP 5'CMV Δtat

    Plasmid
    #101347
    Purpose
    tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.
    Depositor
    Jeremy Luban
    Article
    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:
    Insert
    NL4-3
    Use
    Lentiviral
    Mutation
    delta Tat
    Available Since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    101347-plasmid-map-sequence-id-194257

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  19. pALS2-sfGFP WT

    Plasmid
    #197575
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP WT
    M. alvus Pyl-tRNA (6)
    Tags
    His6
    Expression
    Bacterial
    Promoter
    araC and lpp
    Available Since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197575-plasmid-map-sequence-id-396574

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  20. pAcBac1-Ma-sfGFP WT

    Plasmid
    #197567
    Purpose
    Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP WT
    M. alvus Pyl-tRNA (6) (4x copies)
    Tags
    V5-His6
    Expression
    Mammalian
    Promoter
    CMV and U6/H1
    Available Since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    197567-plasmid-map-sequence-id-389420

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Showing: 541 - 560 of 19273 results