We narrowed to 4,880 results for: pir

1. pRS156

   Plasmid

   #182707

   Purpose

   CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

   Depositor

   Rebecca Shapiro

   Article

   Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SupD v2

   Plasmid

   #198598

   Purpose

   Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SupD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTD103-ARG1

   Plasmid

   #106475

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG1) in Salmonella typhimurium

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLux

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a_T5-ARG1

   Plasmid

   #106476

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015

   Plasmid

   #202023

   Purpose

   Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coli

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al bioRxiv 2024.03.30.587094

   Insert

   Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GvpA T6A-I48V

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. E17TAG GFP zeo resistance

   Plasmid

   #69118

   Purpose

   For E17TAG GFP reporter expression in non-zeocin resistant strains

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   E17TAG GFP
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E17 to TAG

   Promoter

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

7. Beta lactamase S68TAG

   Plasmid

   #68305

   Purpose

   Beta lactamase S68TAG gene

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   S68TAG beta lactamase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_LmFPPS

   Plasmid

   #186322

   Purpose

   pET-28a vector (Novagen) (encoding an N-terminal His tag), to transform Escherichia coli BL21 (DE3) cells

   Depositor

   Sandra Gabelli

   Article

   Aripirala et al Acta Crystallogr D Biol Crystallogr. 201

   Insert

   FPPS
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTcI39-PRM-wasabi

   Plasmid

   #195555

   Purpose

   Temperature-dependent expression of mWasabi reporter with transcription activation by TcI39

   Depositor

   Mikhail Shapiro

   Article

   Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

   Inserts

   mWasabi
   TcI39
   

   Use

   Tags

   6x His and AANDENYADAS degradation tag

   Expression

   Bacterial

   Mutation

   A67T

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015

    Plasmid

    #202024

    Purpose

    Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coli

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al bioRxiv 2024.03.30.587094

    Insert

    Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GvpA T6A-L40A

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAP01

    Plasmid

    #186260

    Purpose

    sfGFP under light light responsive PEL222 promoter

    Depositor

    Yongku Cho

    Article

    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PEL222 (GGTAGCCTTTAGTCCATGTTAGCGAAGAAAATGGTTTGTTA…

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_GEM7-Halo-FRB

    Plasmid

    #197058

    Purpose

    Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1alpha-rtTA-Antares

    Plasmid

    #197587

    Purpose

    Expresses TET-On reverse transactivator labeled with Antares

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    rtTA-P2A-Antares
    

    Use

    Tags

    Antares

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. SQT1665

    Plasmid

    #78744

    Purpose

    Human expression plasmid for LbCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGBW-m4046785

    Plasmid

    #145684

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pJK-caCas9-NatMX-Neut5L

    Plasmid

    #89576

    Purpose

    caCas9 integrating vector into the Neut5L locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHYP-NPC-10H

    Plasmid

    #162789

    Purpose

    SARS-CoV-2 Nucleocapsid Protein expression in E.coli

    Depositor

    Ivan Vorobiev

    Article

    Kolesov et al PeerJ. 2022 Jan 7;10:e12751. doi: 10.771

    Insert

    Nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBEST_OR2-OR1-Pr-UTR1_ClpP-T500

    Plasmid

    #153302

    Purpose

    Expresses ClpP protease in the cell-free TX-TL system

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    clpP (clpP Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMA-Thermal_CTLA-4

    Plasmid

    #199751

    Purpose

    Thermal expression of CTLA-4

    Depositor

    Mikhail Shapiro

    Article

    Abedi et al Nat Commun. 2022 Mar 24;13(1):1585. doi:

    Insert

    CTLA-4 (Ctla4 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGBW-m4046810

    Plasmid

    #149251

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge