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Plasmid#138755PurposeConstitutive expression of VP16-ZF13 under the CMV promoter.DepositorInsertVP16-ZF13
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD547 ZF43x6-Compact_EYFP
Plasmid#138861PurposeEYFP reporter vector for the ZF43x6-Compact promoterDepositorInsertZF43x6-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x6-CompactAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
PCMV-intron myc Rab1aWT
Plasmid#46776Purposeexpression of WT Rab1a in mammalian cellsDepositorInsertRab1a (RAB1A Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PCTK3
Plasmid#20562DepositorInsertPCTK3 (CDK18 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28b-zDjCas13d-His
Plasmid#234481PurposePlasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)DepositorInsertDjCas13d
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPGK-SB13
Plasmid#236078PurposeSB13 transposase, for use with Sleeping Beauty transponson plasmidsDepositorInsertSB13 transposase
UseSleeping beauty (sb) transposase plasmidTagsExpressionMutationPromoterPGKAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNC02
Plasmid#233644PurposeFor bacterial expression of sfGFP c-terminally tagged with VitelloTagB.DepositorInsertsfGFP with c-terminal VitelloTagB
UseTags6-HisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK005
Plasmid#233642PurposeFor bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.DepositorInserteSpCas9(1.1) with c-terminal VitelloTagB
UseTags6-HisExpressionMammalianMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT57
Plasmid#223429PurposeT-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT59
Plasmid#223431PurposeT-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT03
Plasmid#223375PurposeT-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-SpCas9-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT05
Plasmid#223377PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpCas9-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG_GST-TEV-EGFP-ATG13
Plasmid#223797PurposeExpression of recombinant protein for purificationDepositorInsertATG13 (ATG13 Human)
UseTagsGST-TEV-EGFPExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-T4uvsW
Plasmid#228201PurposeExpression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealaseDepositorInsertuvsW
UseTagshexahistidine and 10 amino acid linkerExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Jonah-1
Plasmid#228899PurposeJonah-1 localizes to the outer cyst wall of Acanthamoeba tagged with eGFPDepositorInsertJonah-1
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwn promoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Luke-2
Plasmid#228900PurposeLuke-2 localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFPDepositorInsertCarbohydrate binding domain cbm49 protein
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwnAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Leo-A
Plasmid#228901PurposeLeo-A localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFPDepositorInsertLeo-A
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwnAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRE3G-CTCF-dRBR-HA-MND-zeo
Plasmid#232802PurposeInducible over expression of RNA-binding domain deficient form of CTCF.DepositorInsertCtcf-dRBR (Ctcf Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRE3G-CTCF-WT-HA-MND-zeo
Plasmid#232801PurposeInducible over expression of wild type version of CTCF.DepositorInsertCTCF (Ctcf Mouse)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1_puro_shNRF2-1
Plasmid#230086PurposeshRNA silencing human NRF2 geneDepositorInsertNrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only