We narrowed to 17,461 results for: She

1. Nbr_C_sgRNA1

   Plasmid

   #186663

   Purpose

   Nbr C-tag sgRNA1 plasmid

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   Nbr sgRNA 1 Plasmid (Nbr Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Shu_sgRNA

   Plasmid

   #186656

   Purpose

   shu sgRNA plasmid

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   Shu sgRNA Plasmid (shu Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Cas9_nonPuro_OSS

   Plasmid

   #186652

   Purpose

   Cas9 plasmid to express the Cas9 in OSS cells

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Cas9 Expression Plasmid
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. VIR_sgRNA

   Plasmid

   #186661

   Purpose

   VIR sgRNA plasmid

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   VIR sgRNA Plasmid (vir Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCEP4_HLA-cmyc-GRM3 C127A T174A-VC

   Plasmid

   #140457

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and T174A mutant fused to the C-terminal half of split fluorescent Venus (VC)

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine, Threonine 174 to alanine

   Promoter

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLX_HA-C7orf26v2

   Plasmid

   #178536

   Purpose

   Subcloned C7orf26v2 (ENST00000359073, Broad GPP TRCN0000477172) into pLX_HA backbone

   Depositor

   William Hahn

   Article

   Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

   Insert

   C7orf26v2 (INTS15 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HEpl016

   Plasmid

   #179991

   Purpose

   pCBh_3xFLAG_NLS_SiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized SiCas12i driven by CBh promoter and tagmCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   SiCas12i
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQC V5 mKO2 HuR.wt IRES Puro

   Plasmid

   #110387

   Purpose

   γ-Retroviral transfer vector for expressing HuR (wild type), V5 and mKO2 tags, IRES-driven Puromycin selection.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Retroviral

   Tags

   V5 and mKO2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT5T_Rev7_1-211_SA112_KVK_Champ1_328-355

   Plasmid

   #105638

   Purpose

   Expresses mouse monomeric Rev7 and fragment of Champ1 protein (amino acids 328-355) from bicistronic plasmid

   Depositor

   Thanos Halazonetis

   Inserts

   Rev7 (Mad2l2 Mouse)
   Champ1 (CHAMP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutations: F11S, G12A, V132K, C133V, A135K

   Promoter

   T7

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLX_HA-C7orf26v1

    Plasmid

    #178535

    Purpose

    Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_HA backbone

    Depositor

    William Hahn

    Article

    Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

    Insert

    C7orf26v1 (INTS15 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330 NLC-FKBP-Cas9

    Plasmid

    #162726

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330 C-FKBP-Cas9

    Plasmid

    #162729

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSIN-Gapdh

    Plasmid

    #175938

    Purpose

    knockout mouse Gapdh

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Gapdh gRNA (Gapdh Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQC hKGA.S286A V5-His IRES G418

    Plasmid

    #110391

    Purpose

    γ-Retroviral transfer vector for expressing glutminase isoform KGA (S286A), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    KGA glutaminase (GLS Human)
    

    Use

    Retroviral

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    S286A

    Promoter

    CMV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV_eA3A T31A-nCas9

    Plasmid

    #163538

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A T31A-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)eA3A T31A

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV_UdgX-EE-UdgX-nCas9

    Plasmid

    #163564

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. myc-Miro2 (lacking TM)

    Plasmid

    #127614

    Purpose

    Human Miro2 with the transmembrane deleted

    Depositor

    Josef Kittler

    Article

    Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

    Insert

    Miro2 (RHOT2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Transmembrane deletion

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSR357.12

    Plasmid

    #125107

    Purpose

    Expression of narL(1-134aa)-ydfI(129-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)134
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    A84T - see depositor comments below and Q183K-see…

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-deltaA1-R1597W-VWF

    Plasmid

    #124826

    Purpose

    Expresses multimeric human VWF protein without A1 domain and a R1597W mutation in A2 domain. This protein has C-terminal c-Myc tag.

    Depositor

    Sriram Neelamegham

    Article

    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

    Insert

    VWF without A1 domain and R1597W mutation A2 domain (VWF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-deltaA1-D1498A-VWF

    Plasmid

    #124825

    Purpose

    Expresses multimeric human VWF protein without A1 domain and a D1498A mutation in A2 domain. This protein has C-terminal c-Myc tag.

    Depositor

    Sriram Neelamegham

    Article

    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

    Insert

    VWF without A1 domain and D1498A mutation A2 domain (VWF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Myc-gamma3/gamma2SIL

    Plasmid

    #121433

    Purpose

    GABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3/GABRG2 SIL (Gabrg2, Gabrg3 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Myc-gamma1/gamma2SIL

    Plasmid

    #121432

    Purpose

    GABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG1/GABRG2 SIL (Gabrg1, Gabrg2 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX6P-1 PUM FL

    Plasmid

    #112997

    Purpose

    For protein expression and purification of Drosophila pumilio (amino acids 566-1090)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    pumilio (pum Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET151-Vps4(101-437)-Hcp1 K321D

    Plasmid

    #89288

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Lys-321 to Asp

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET151-Vps4(101-437)-Hcp1 R325D

    Plasmid

    #89289

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Arg-325 to Asp

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET151-Vps4(101-437)-Hcp1 E322A

    Plasmid

    #87748

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Glu-322 to Ala

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET151-Vps4(101-437)-Hcp1 R325A

    Plasmid

    #87749

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Arg-325 to Ala

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET151-Vps4(101-437)-Hcp1 K321A

    Plasmid

    #87747

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Lys-321 to Ala

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. csd2_121-308,S166A/pET28b(+)

    Plasmid

    #83301

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag, mutation: S166A

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120, and changed Serine 166 …

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. csd2_121-308,T151A/pET28b(+)

    Plasmid

    #83300

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag, mutation: T151A

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120, and changed Threonine 1…

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. csd2_121-308,Y197F/pET28b(+)

    Plasmid

    #83299

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag, mutation: Y197F

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120, and changed Tyrosine 19…

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. csd2_121-308,E165A/pET28b(+)

    Plasmid

    #83298

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag, mutation: E165A

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120, and changed Glutamic ac…

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. GST-CHMP4A (205-222) (pGEX2T(TEV))

    Plasmid

    #80633

    Purpose

    Residues 205-222 of CHMP4A, Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 205-222;

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. csd6_4-330/pET28b(+) N-tag

    Plasmid

    #71351

    Purpose

    csd6, construct:4-330, pET28b(+) N-tag

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 )
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

35. csd6_13-330/pET28b(+) N-tag

    Plasmid

    #71352

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 )
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

36. csd6_1-330/pET28b(+) N-tag

    Plasmid

    #71350

    Purpose

    csd6, construct:1-330, pET28b(+) N-tag

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 )
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

37. sgGLDC_2

    Plasmid

    #72887

    Purpose

    CRISPR-Cas9 induced gene disruption

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    sgGLDC
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

38. sgGLDC_1

    Plasmid

    #72886

    Purpose

    CRISPR-Cas9 induced gene disruption

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    sgGLDC
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

39. pBabe_puro_DEST_Flag_LRRC31

    Plasmid

    #45271

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    LRRC31 (LRRC31 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pCDF1-ADAMTS18 (P1035S)

    Plasmid

    #29558

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed proline 1035 to serine

    Promoter

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only