We narrowed to 23,387 results for: Kars

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pETM6-T7-VHH-GFPminimizer

Plasmid

#202488

Purpose

Expresses the nanobody GFP minimizer with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP minimizer

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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BPK2301

Plasmid

#65778

Purpose

Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

St1Cas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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MSP1594

Plasmid

#65775

Purpose

Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shFEN

Plasmid

#17921

Depositor

Sheila Stewart

Article

Saharia et al Curr Biol. 2008 Apr 8. 18(7):496-500.

Insert

shFEN (FEN1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pKERep

Plasmid

#211826

Purpose

Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pKESa

Plasmid

#211828

Purpose

Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCK364.306

Plasmid

#192642

Purpose

Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

J306 scRNA

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pUb dPspCas13b + PspCas13b guide RNA

Plasmid

#176306

Purpose

Expresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

PspCas13b

Tags

HA tag

Expression

Insect

Mutation

H133A, H1058A

Promoter

Ubi-p63e

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_039 FnCas12a

Plasmid

#171666

Purpose

For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

FnCas12a

Tags

6X His and sortase A sorting motif

Expression

Bacterial

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-nptII

Plasmid

#165876

Purpose

Basic G418 resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pNK6032

Plasmid

#219738

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter FMV

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

Figwort mosaic virus (FMV) promoter

Use

Synthetic Biology

Expression

Plant

Promoter

FMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_SNAP

Plasmid

#82716

Purpose

Expresses Myosin VI HMM with a C-terminal SNAP tag.

Depositor

Sivaraj Sivaramakrishnan

Article

Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

Insert

Myosin VI

Tags

6xHis Tag, FLAG purification tag, and SNAP tag

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV_HaloK73T_cyto

Plasmid

#183687

Purpose

Mammalian expression of cytosolic metastable Halotag/folding sensor (Cyto HaloK73T)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

Cyto Halo(K73T)

Expression

Mammalian

Mutation

K73T in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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Psicheck2 PTPRN2 full length 3'UTR

Plasmid

#26994

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

PTPRN2 Full length 3' UTR (PTPRN2 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pRS415_pGal-nCas9(H840A)

Plasmid

#79616

Purpose

Expresses nCas9(H840A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

H840A for nickase Cas9

Promoter

pGal1

Available Since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-FCU1

Plasmid

#165882

Purpose

Basic 5-fluorocytosine sensitivity cassette in Alpha2, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pC002 - LshC2C2 locus into pACYC184

Plasmid

#79151

Purpose

Endogenous LshC2c2 locus cloned into pACYC184

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

LshC2c2 locus

Expression

Bacterial

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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pSMP-Suv39H1_1

Plasmid

#36342

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Suv39H1 (SUV39H1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSLQ5135: pCAG-BFP-GuideArray

Plasmid

#210606

Purpose

Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

pCAG-BFP-GuideArray

Use

CRISPR

Promoter

CAG

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-Pidd1(T788A)

Plasmid

#60529

Purpose

Retroviral expression of Pidd1 T788A phosphorylation mutant

Depositor

Trudy Oliver

Article

Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.

Insert

Pidd1 (PIDD1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Threonine 788 to Alanine*

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only