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anti-Notch2_B9-pBIOCAM5

Plasmid

#39348

Depositor

John McCafferty

Article

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch2 (clone B9) (NOTCH2, Notch2 Human, Mouse)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(E123A)-mCherry

Plasmid

#35506

Purpose

AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123A

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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Lenti-sgApc/Cre

Plasmid

#89641

Purpose

Expresses an Apc-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Apc (Apc Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEntry_MERS-CoV-nsp3

Plasmid

#168838

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-nsp3 (orf1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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hsp70: mKate2-DN Kif5a

Plasmid

#105972

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

Kif5a (kif5aa Zebrafish)

Tags

mKate2

Expression

Bacterial

Mutation

truncation of aa416-1033

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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-227 hTF (mutant AP-1)

Plasmid

#15444

Depositor

Nigel Mackman

Article

Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Both AP-1 sites mutated

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
pLVX Flag RagC W115R

Plasmid

#112759

Purpose

express Flag-RagC (W115R mutant)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (W115R) (RRAGC Human)

Use

Lentiviral

Tags

Flag

Mutation

W115R

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSR357.24

Plasmid

#125109

Purpose

Expression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)159
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX Flag RagC T90N

Plasmid

#112758

Purpose

express Flag-RagC (T90N mutant)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (T90N) (RRAGC Human)

Use

Lentiviral

Tags

Flag

Mutation

T90N

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_PAX7_P2A_Hygro_Barcode

Plasmid

#120472

Purpose

Barcoded lentiviral vector to express PAX7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

PAX7 (PAX7 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-myc-BioID-hp100

Plasmid

#174738

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Article

Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

Insert

P100 (NFKB2 Human)

Use

Retroviral

Mutation

WT

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLXSN-H-Ras_N17

Plasmid

#39537

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS N17 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

N17

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pEntry_MERS-CoV-Nucleocapsid

Plasmid

#168833

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-Nucleocapsid (N )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_ONECUT1_P2A_Hygro_Barcode

Plasmid

#120470

Purpose

Barcoded lentiviral vector to express ONECUT1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ONECUT1 (ONECUT1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_SOX3_P2A_Hygro_Barcode

Plasmid

#120481

Purpose

Barcoded lentiviral vector to express SOX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX3 (SOX3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-sgKeap1/Cre

Plasmid

#89645

Purpose

Expresses a Keap1-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Keap1 (Keap1 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Plasmid

#217513

Purpose

Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_C_LAMP1

Plasmid

#111669

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

LAMP1 (LAMP1 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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IRAK1mut-UTR

Plasmid

#15096

Depositor

David Baltimore

Article

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

IRAK1 3'UTR (IRAK1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

4-bp substitutions in the miR-146 target sites

Available Since

June 6, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-CaMKIIa-eArch 3.0-EYFP

Plasmid

#35514

Purpose

Lentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eArch3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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