We narrowed to 59,538 results for: cloning

1. GGE255

   Plasmid

   #120788

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp_Lip-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p663-UBC-V5-BRSK2_IDG-K

   Plasmid

   #135273

   Purpose

   Gateway destination clone of BRSK2 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   V5-BRSK2 (BRSK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p663-UBC-miniTurbo-V5-GSK3B_IDG-K

   Plasmid

   #158165

   Purpose

   Gateway destination clone of GSK3B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-GSK3B (GSK3B Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5/FRT/TO HIS DNAJB2b

   Plasmid

   #19551

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB2b
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFOSGE_COF2F12

   Plasmid

   #71060

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Chd3 (Chd3 Fly)
   

   Use

   Gateway entry vector

   Available Since

   Dec. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pFOSGE_COF2D09

   Plasmid

   #71017

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   MED15 (MED15 Fly)
   

   Use

   Gateway entry vector

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pFOSGE_COF3D10

   Plasmid

   #71004

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   MED23 (MED23 Fly)
   

   Use

   Gateway entry vector

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pFOSGE_COF1B02

   Plasmid

   #70782

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   MED9 (MED9 Fly)
   

   Use

   Gateway entry vector

   Available Since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pKJ-1

   Plasmid

   #11333

   Depositor

   Michael McBurney

   Article

   Adra et al Gene. 1987 . 60(1):65-74.

   Insert

   pgk-1 5' and 3' regions (Pgk1 Mouse)
   

   Expression

   Bacterial

   Available Since

   March 10, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pENTR pA L3-L2

    Plasmid

    #43966

    Depositor

    Peter Hohenstein

    Article

    Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

    Insert

    pA
    

    Use

    Multisite gateway entr clone

    Available Since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5/FRT/TO HIS DNAK

    Plasmid

    #19544

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    dnaK molecular chaperone DnaK
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMBD-Gate3

    Plasmid

    #25959

    Depositor

    Kamil Onder

    Article

    Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

    Insert

    
    

    Tags

    GAL4 Fusion Protein

    Expression

    Mammalian

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p663-UBC-miniTurbo-V5-ICK_IDG-K

    Plasmid

    #158187

    Purpose

    Gateway destination clone of ICK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-ICK (CILK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GGE270

    Plasmid

    #120780

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Coding sequence of fluorescent protein in position 1 (G1_YFP)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    G1_YFP
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pICSL80014

    Plasmid

    #50320

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, YFP variant of GFP (A. victoria)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5/FRT/TO GFP DNAJB3

    Plasmid

    #19498

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB3
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFOSGE_COF3F06

    Plasmid

    #70920

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Orc4 (Orc4 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

18. BSP179

    Plasmid

    #110915

    Purpose

    For cloning expression cassettes by HR in yeast, Universal MosSCI compatible

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Yeast homologous recombination…

    Expression

    Worm

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pME-nEGFP

    Plasmid

    #138363

    Purpose

    Gateway compatible middle entry clone contains nuclear EGFP sequences.

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    nEGFP
    

    Expression

    Mammalian

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pME-6MTzactMap2k2b

    Plasmid

    #67710

    Purpose

    Middle entry clone

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    activated Map2k2b (map2k2b Zebrafish)
    

    Mutation

    S221D, S225D

    Promoter

    none

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge