We narrowed to 10,236 results for: ada

1. pNCST-mAvicFP1

   Plasmid

   #129509

   Purpose

   Expresses mAvicFP1 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   mAvicFP1
   

   Expression

   Bacterial

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221_SLC39A8

   Plasmid

   #132177

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

   Insert

   SLC39A8 (SLC39A8 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAAV_hSyn1_AchLightR

   Plasmid

   #187463

   Purpose

   Expresses red acetylcholine indicator AchLightR in neuronal cells

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Insert

   AchLightR
   

   Use

   AAV

   Tags

   Igκ leader

   Promoter

   human Synapsin-1

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKK-TEV-3XFLAG

   Plasmid

   #105784

   Purpose

   Expression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-3XFLAG

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNH-TrxT-G-S18

   Plasmid

   #127827

   Purpose

   Production of recombinant human ribosomal protein S18

   Depositor

   Ivanhoe Leung

   Article

   Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

   Insert

   RPS18 (RPS18 Human)
   

   Tags

   His6 - Thioredoxin - TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMonAID_dCas9-PmCDA_Hyg_ALS

   Plasmid

   #91692

   Purpose

   Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A and H840A for dead Cas9

   Promoter

   2x35S

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-ACBEmax

   Plasmid

   #139106

   Purpose

   Mammalian ACBEmax expression vector (pSI-632)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO-ChroME-ST-P2A-H2B-mRuby3

   Plasmid

   #170161

   Purpose

   Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME-P2A-H2B-mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNH605-CCAAT-YFP

   Plasmid

   #172132

   Purpose

   Fluorescent reporter for HAP complex activity

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   4xCCAAT-YFP
   

   Use

   Vector for genomic integration into the yeast gen…

   Expression

   Yeast

   Promoter

   CYC1 core promotor with 4 tandem repeats of the H…

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE2-TetOminiCMV-Sox2

    Plasmid

    #136612

    Purpose

    Dox-inducible lentiviral vector expressing mouse Sox2

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_eA3A T31A-dCas9

    Plasmid

    #163537

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A T31A-dCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    dCas9 (D10A, H840A)eA3A T31A

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE2-TetOminiCMV-cMyc

    Plasmid

    #136614

    Purpose

    Dox-inducible lentiviral vector expressing mouse cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    cMyc (Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJ4M_hnRNPA2_FL_D290V

    Plasmid

    #139110

    Purpose

    Express FL hnRNPA2 D290V with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Expression

    Bacterial

    Mutation

    D290V

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1P POLE1_1

    Plasmid

    #160762

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-PRAS40 AA

    Plasmid

    #86759

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    human PRAS40 (AKT1S1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AA (S183A, S221A)

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. cytosolic moPrP (3F4)

    Plasmid

    #1321

    Depositor

    Susan Lindquist

    Article

    Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

    Insert

    PrP (Prnp Mouse)
    

    Expression

    Mammalian

    Mutation

    N-term truncation (to make gene cytosolic), vario…

    Available Since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3 βarr1 S412D Flag

    Plasmid

    #42196

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    β-arrestin 1 S412D (Arrb1 Rat)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S412D

    Promoter

    CMV

    Available Since

    Jan. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid

    #141236

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    NES-jRGECO1a
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    6xHIS tag and nuclear export signal

    Promoter

    human elongation factor-1 alpha (EF-1 alpha)

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-hIP-dCas9-BSD

    Plasmid

    #183231

    Purpose

    Lentiviral vectors with the human insulin promoter driving expression of dCas9, in addition to a U6-driven sgRNA

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    Human insulin promoter

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT5T_hp53_ST

    Plasmid

    #34922

    Depositor

    Thanos Halazonetis

    Article

    Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

    Insert

    p53 DNA-binding (Res 1-393) (TP53 Human)
    

    Expression

    Bacterial

    Mutation

    C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

    Promoter

    T7

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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