We narrowed to 5,844 results for: pcas

1. pLV-TET1-dCas9

   Plasmid

   #235599

   Purpose

   Expresses Tet1CD-dCas9

   Depositor

   Ting Zhou

   Article

   Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

   Insert

   TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xNLS and TET1 CD

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYO1015

   Plasmid

   #235704

   Purpose

   Expression of FL human VPS15-ZZ in mammalian cells

   Depositor

   Roger Williams

   Article

   Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

   Insert

   VPS15 (PIK3R4 Human)
   

   Tags

   3xTEV-ZZ

   Expression

   Mammalian

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYO1005

   Plasmid

   #235702

   Purpose

   Expression of FL human UVRAG in mammalian cells

   Depositor

   Roger Williams

   Article

   Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

   Insert

   UVRAG (UVRAG Human)
   

   Expression

   Mammalian

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. R2pH-LAMP-FRB

   Plasmid

   #225138

   Purpose

   R2pH-LAMP1-labelled lysosomes with FRB

   Depositor

   Massimiliano Stagi

   Article

   Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

   Insert

   Lamp1 (Lamp1 Mouse)
   

   Expression

   Mammalian

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. TadA-N46L-NG-CBE

   Plasmid

   #227419

   Purpose

   C-to-T base editing in mammalian cells

   Depositor

   Yang Sun

   Article

   Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

   Insert

   TadA-N46L-nSpCas9-NG-2xUGI (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. YEE-NG-CBE

   Plasmid

   #227418

   Purpose

   C-to-T base editing in mammalian cells

   Depositor

   Yang Sun

   Article

   Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

   Insert

   rA1-YEE-nSpCas9-NG-2xUGI (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgRNA1_Cas9-hGem_Smc4

   Plasmid

   #217667

   Purpose

   expresses Cas9-hGem and guideRNA for tagging of SMC4

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   sgRNA1 targeting SMC4 C-terminal (SMC4 Human)
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH

   Plasmid

   #131684

   Purpose

   An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1

   Depositor

   Christopher Richie

   Article

   Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

   Inserts

   EGFP-KASH
   SpCas9 sgRNA vs mouse GRID1
   

   Use

   AAV

   Tags

   KASH

   Promoter

   EF1a and mU6-LSL (Cre dependent)

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a-hP53DD (TET)

   Plasmid

   #214083

   Purpose

   Mammalian expression tetramerization domain (TET) of human TP53

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   hP53DD (TP53 Human)
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. kiCAP-AAV-BI-hTFR1-4

    Plasmid

    #218799

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al Science. 2024 Jun 14;384(6701):1220-1227

    Insert

    AAV Rep-Cap BI-hTFR1-4
    

    Expression

    Mammalian

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. B2M-SDMutation-gRNA

    Plasmid

    #215547

    Purpose

    gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    B2M (B2M Human)
    

    Use

    CRISPR

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGMC00036 (aka pMC0223)

    Plasmid

    #212659

    Purpose

    Guide RNA targeting SERPINB3

    Depositor

    Raj Chari

    Article

    Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

    Insert

    SERBPINB3 guide RNA (SERPINB3 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. INS-3'gRNA

    Plasmid

    #210468

    Purpose

    gRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    INS (INS Human)
    

    Use

    CRISPR

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

    Plasmid

    #208977

    Purpose

    Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

    Plasmid

    #208974

    Purpose

    Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

    Plasmid

    #208971

    Purpose

    Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

    Plasmid

    #208970

    Purpose

    Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29(+exo)-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. UAS-dsarm(E,K450R)::flag

    Plasmid

    #187882

    Purpose

    Gal4/UAS expression of dSarm(isoE,K450R)::FLAG

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    dSarm isoform E (Sarm )
    

    Tags

    FLAG

    Mutation

    K450R

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. UAS-dsarm(E)::flag

    Plasmid

    #187871

    Purpose

    Gal4/UAS expression of dSarm(isoE)::FLAG

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    dSarm isoform E (Sarm Fly)
    

    Tags

    FLAG

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. TKIT NFL gRNA1 gRNA2

    Plasmid

    #182681

    Purpose

    Expression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    two Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 and chicken beta-actin promoter

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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