We narrowed to 15,991 results for: LEA

1. pAAV-GfaABC1D-GRAB_g5-HT3.0

   Plasmid

   #208727

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytes

   Depositor

   Yulong Li

   Article

   Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0
   

   Use

   AAV

   Promoter

   GfaABC1D

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-JNK1

   Plasmid

   #86830

   Purpose

   mammalian expression of GFP-JNK1

   Depositor

   Rony Seger

   Article

   Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

   Insert

   JNK1 (MAPK8 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dominant negative Hsp90 beta

   Plasmid

   #22480

   Depositor

   William Sessa

   Article

   Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

   Insert

   Dominant negative Hsp90 (HSP90AB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Dec. 8, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

   Plasmid

   #86421

   Purpose

   N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   CDC42pr-KAR2SS-GFP1-10
   

   Tags

   GFP1-10

   Expression

   Yeast

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICE-HA-MRE11-H63S

   Plasmid

   #82035

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   MRE11 (MRE11 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H63S MRE11 mutant

   Promoter

   CMV-tet

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-EGxxFP-Cetn1

   Plasmid

   #50717

   Purpose

   Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

   Depositor

   Masahito Ikawa

   Article

   Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

   Insert

   Cetn1 (Cetn1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. AY27_pU6.gRNA.CLYBL

   Plasmid

   #199238

   Purpose

   Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   S. pyogenes gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cas12j-8-puro-crRNA

   Plasmid

   #194965

   Purpose

   The plasmid expresses human codon-optimized Cas12j-8, crRNA expression elements and puromycin resistence gene.

   Depositor

   Yongming Wang

   Article

   Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hsyn-GRAB_Ado1.0med

   Plasmid

   #153286

   Purpose

   Express the medium-affinity adenosine sensor GRAB_Ado1.0med in neurons

   Depositor

   Yulong Li

   Article

   Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Adenosine sensor GRAB_Ado1.0med (medium affinity)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDisplay-GRAB_OT1.0

    Plasmid

    #185384

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin sensor GRAB_OT1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-REPORT(PGKCMV)-nucTomato-hygro

    Plasmid

    #208573

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    mnucTomato
    d2nucEGFP
    HygroR
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK/CMV and minP

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-BirA*-pDEST-N-pcDNA5/FRT/TO

    Plasmid

    #118375

    Purpose

    Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    HA-BirA*

    Expression

    Mammalian

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICSL11059

    Plasmid

    #68263

    Purpose

    Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPBbsr2-Raichu-454HRasCT

    Plasmid

    #176029

    Purpose

    A FRET biosensor for HRas activity

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    Raichu-Ras-HRasCT
    

    Expression

    Mammalian

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWF079_LVt-EF1a-TCRGibson_humanTRAC

    Plasmid

    #233448

    Purpose

    Gibson assembly of TCRB-P2A-TCRa

    Depositor

    William Freed-Pastor

    Article

    Ely et al Science. 2025. May 8; 388(6747)

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-BHMT-I/G

    Plasmid

    #104442

    Purpose

    Autophagy cargo assay

    Depositor

    Carol Mercer

    Article

    Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

    Insert

    BHMT (BHMT Human)
    

    Tags

    GFP-myc GFP is from Aequorea victoria and GST

    Expression

    Mammalian

    Mutation

    N43A in GST (in a potential KFERQ-like sequence).…

    Promoter

    CMV

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b CnA CnB

    Plasmid

    #11787

    Depositor

    Anjana Rao

    Article

    Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-4

    Insert

    CnA CnB (PPP3R1, PPP3CA Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 4, 2006

    Availability

    Academic Institutions and Nonprofits only

18. YPET-GGA3

    Plasmid

    #18841

    Depositor

    Martin Schwartz

    Article

    Hall et al Anal Biochem. 2008 Mar 15. 374(2):243-9.

    Insert

    YPET-GGA3 (GGA3 Human)
    

    Tags

    His6 and myc

    Expression

    Mammalian

    Mutation

    The fluorescent protein YPET was added to the ami…

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

19. Ad-Flag-XBP1s

    Plasmid

    #63678

    Purpose

    Adenoviral vector for XBP1s expression

    Depositor

    Lingfang Zeng, Qingbo Xu

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    spliced

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

    Plasmid

    #226009

    Purpose

    DjCas13d guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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