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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,189 results for: LEA

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Showing: 5621 - 5640 of 12189 results

  2. O-MARS-11

    Plasmid
    #166541
    Purpose
    scFv of a human scaffold targeting Methionyl-tRNA synthetase. Antigen coverage aa 1-225 of 900
    Depositor
    Susanne Gräslund
    Publication
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166541-plasmid-map-sequence-id-329234

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  3. L-YARS2-9

    Plasmid
    #166533
    Purpose
    scFv of a human scaffold targeting Tyrosyl-tRNA synthetase 2, mitochondrial. Antigen coverage aa 31-477 of 477
    Depositor
    Susanne Gräslund
    Publication
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166533-plasmid-map-sequence-id-329205

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  4. L-SARS-7

    Plasmid
    #166527
    Purpose
    scFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-length
    Depositor
    Susanne Gräslund
    Publication
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166527-plasmid-map-sequence-id-329199

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  5. L-GARS-2

    Plasmid
    #166531
    Purpose
    scFv of a human scaffold targeting Glycyl-tRNA synthetase. Antigen coverage aa 55-739 of 739
    Depositor
    Susanne Gräslund
    Publication
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166531-plasmid-map-sequence-id-328315

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  7. pGBW-m4134196

    Plasmid
    #152506
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152506-plasmid-map-sequence-id-352067

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  8. pGBW-m4134452

    Plasmid
    #152327
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp2
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152327-plasmid-map-sequence-id-317257

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  9. pGBW-m4133873

    Plasmid
    #152210
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp10
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;TRX
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152210-plasmid-map-sequence-id-389777

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  10. pGBW-m4133361

    Plasmid
    #154789
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 ORF6 protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154789-plasmid-map-sequence-id-307385

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  11. pGBW-m4134333

    Plasmid
    #152671
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 helicase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152671-plasmid-map-sequence-id-300224

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  12. pGBW-m4134071

    Plasmid
    #152106
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152106-plasmid-map-sequence-id-300777

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  13. pGBW-m4133221

    Plasmid
    #152141
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 helicase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152141-plasmid-map-sequence-id-297951

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  17. pOCC94

    Plasmid
    #118912
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Tags
    MBP, cleavable with 3C protease and eGFP-HIS6
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118912-plasmid-map-sequence-id-232258

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  18. pICSL11023

    Plasmid
    #117542
    Purpose
    Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Publication
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
    Expression
    Plant
    Promoter
    2x35S_OMEGA(pICH51288)
    Available since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    117542-plasmid-map-sequence-id-228061

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  19. 5WBN (HDAC6)

    Plasmid
    #101267
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HDAC6 (HDAC6 Human)
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101267-plasmid-map-sequence-id-193501

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  20. p5B3-T

    Plasmid
    #92021
    Purpose
    Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Publication
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lysA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Mutation
    S219V
    Available since
    June 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    92021-plasmid-map-sequence-id-181444

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Showing: 5621 - 5640 of 12189 results