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We narrowed to 9,887 results for: crispr plasmids

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Showing: 5641 - 5660 of 9887 results

  5. OmEF1aCas9P2ApuroSB

    Plasmid
    #165484
    Purpose
    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells
    Depositor
    Dietmar Kueltz
    Article
    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10
    Insert
    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    Use
    CRISPR; Transposon
    Promoter
    Oreochromis mossambicus EF1 alpha promoter (OmEF1…
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    165484-plasmid-map-sequence-id-330622

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  6. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  8. pCSN061

    Plasmid
    #101725
    Purpose
    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Article
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available Since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    101725-plasmid-map-sequence-id-199888

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  9. RFN_EGFP_81

    Plasmid
    #60073
    Purpose
    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
    Depositor
    Keith Joung
    Article
    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    pair of multiplex gRNAs targeting EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. MSP2266

    Plasmid
    #70705
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70705-plasmid-map-sequence-id-131909

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  20. pCas9GG

    Plasmid
    #131009
    Purpose
    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Bba_R0040 TetR
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131009-plasmid-map-sequence-id-257082

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Showing: 5641 - 5660 of 9887 results